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Cysteine (CYS) content test kit (colorimetric method) R200413 Basic information More.. Product Name: Cysteine (CYS) content test kit (colorimetric method) R200413 Synonyms: Cysteine (CYS) content test kit (colorimetric method) R200413 CAS: MF: MW: 0 EINECS: Mol File: Mol File ...
Fig. 1. Effects of Tyr- and Cys-containing tripeptides on MPO-catalyzed HOCl formation. MPO (20 nM) was incubated with H2O2 (50 μM), NaCl (150 mM), taurine (5 mM), and various amounts of tripeptides in phosphate buffer (100 mM, pH 7.4) containing DTPA (100 μM) for 30 min....
The induction of cys biosynthesis genes and elevated GSH in actively migrating swarm cells suggests the swarm cells should show elevated resistance to oxidative stress. We used a disk diffusion assay to measure resistance to H2O2. Swarm cells had no zones of inhibition and migrated to the edge of...
In this study, antibacterial surfaces with a "disperse-then-kill" synergy were prepared by embedding d -cysteine ( d -cys) functionalised silver nanoparticles (AgNPs) onto polydopamine-coated stainless steel. The as-prepared surface exhibited excellent antibacterial performance against the gram-negative...
Finally, we compared the ATP-dependent production of folded Rhodanase when bound by MmCpn•Cys-0 versus MmCpn WT (Fig.3D). Rhodanese folding results in active enzyme that can be measured using a colorimetric assay. The rhodanese-Cpn complex was incubated in the absence or presence of ATP ...
The H2O2produced during holo-SOD1WT-catalyzed thiol oxidation would increase the oxidizing potential of the protein’s immediate environment, potentially affecting redox-sensitive surface-exposed groups of the protein. The SOD1 contains a highly conserved intramolecular disulfide bond (Cys57-Cys146), wh...
Peptide concentrations were obtained through the use of the Pierce Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific, Cat no. 23275). Manufacturer's protocol was followed. Assessing bead concentration and size A Thermo Fisher Countess II was used to measure bead concentration and size for...
The colorimetric substrate CellTiter 96® AQueous One (Promega) was added 18 h before the end of the assay. All measurements were carried out at 492 nm on a Multiskan Ascent plate reader (Thermo Scientific). Cells and medium alone were used as negative controls. Background medium measurements...
Cysteine (CYS) content test kit (colorimetric method) Basic information More.. Product Name: Cysteine (CYS) content test kit (colorimetric method) Synonyms: Cysteine (CYS) content test kit (colorimetric method) CAS: MF: MW: 0 EINECS: Mol File: Mol File Information Error Report Your ...