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Home > Trace elements > Cu / Copper assayRev.150627 Easy to use. For cell lysates, serum, plasma and wide variety of biological samples. Pre-preatment free! Species independent! No toxic substance! Applicable to microplate reader! [ Product Description ] Inside human body, approximately 95% o...
The copper colorimetric assay kit was purchased from Chuangye biological Ltd.. (Shangyu, China). 2.2. Instrumentation Fluorescence and UV–visible spectra were obtained using a Fluorescence Max-P fluorometer (HORIBA JobinYvon Inc., USA) and a Cary 5000 spectrophotometer (Varian, USA), respectively...
(brown fits,Fig. 2b; and brown histogram,Fig. 2d). The absence of the∼6nm intermediate in the AzuC112Acase firmly suggests that the∼6nm intermediate observed in the unfolding trajectories of wildtype (wt)-Azu (Fig. 1d) is indeed triggered by the mechanical disruption of the Cu–S...
[55]. Since ceruloplasmin in serum might be correlated to Cu concentrations, we determined these levels using a Ceruloplasmin Colorimetric Activity Kit (Invitrogen, Waltham, MA). The hemoglobin levels were measured using a hemoglobin assay kit (Chondrex Inc., Woodinville, WA), which correlates with...
and SOD activities were measured by guaiacol, spectrophotometry, and nitro blue tetrazolium methods, respectively. Proline and MDA contents were measured using the ninhydrin reaction and thiobarbituric acid colorimetric methods, respectively. Last, the 2,3,5-triphenyltetrazolium chloride (TTC) method was...
A) Enzymatic activity of Cu,Zn-SOD. B) Enzymatic activity of Mn-SOD. C) Enzymatic activity of CAT. The enzymatic activities were determined in lysated cells of control and treated cells with Cas III-ia for the techniques described in methods section. Data representative of means ± SD...
The supernatant was used for copper content determination with the Cell Copper (Cu) Colorimetric Assay Kit. Refer to the instructions provided for specific procedure steps. For the heat-killed control group, pre-cultured strains were washed with metal-ion-free PBS and suspended in sterile deionized...
(O2) to H2O2. A colorimetric method was used to measure H2O2generation from the oxidation reaction between H2O2and KMnO4by the observation of the drop of the absorption band at 525 nm contributed from KMnO4(Fig.2a). The absorbance intensity of 525 nm decreased, indicating H2O2generation, ...
Cell viability was evaluated using colorimetric MTS assay that measures cellular metabolic activity. Cells were seeded into 96-well plates at 5 × 103 cells per well in 80 μl full medium for 24 h, after that Complex (50 µM), Ligand (50 µM and 100 µM), DMSO ...