Home > Trace elements > Cu / Copper assayRev.150627 Easy to use. For cell lysates, serum, plasma and wide variety of biological samples. Pre-preatment free! Species independent! No toxic substance! Applicable to microplate reader! [ Product Description ] Inside human body, approximately 95% o...
The copper colorimetric assay kit was purchased from Chuangye biological Ltd.. (Shangyu, China). Instrumentation Fluorescence and Fluorescence properties of AuNCs@DTT The excitation and emission wavelengths of the DTT-capped gold nanoclusters were 330 and 604 nm, respectively (Fig. 1b). During the...
Cell membrane integrity was evaluated by [28] using a colorimetric LDH cytotoxicity assay kit (KLDH96,Kiazist, Iran). MRC-5 cells were seeded in 96-well microplates (12.5 × 104 cells/well) and incubated for 24 h. Cells were then treated with Cu-doped ZnO NPs, ZnO NPs, and bulk...
26]. We comprehensively characterized these NPs and evaluated their biological activity, comparing their in vitro and in vivo cytotoxicity to both undoped ZnO NPs and bulk ZnO. This is the first study to investigate the cancer-preventive properties of Cu-doped ZnO NPs, using...
Since ceruloplasmin in serum might be correlated to Cu concentrations, we determined these levels using a Ceruloplasmin Colorimetric Activity Kit (Invitrogen, Waltham, MA). The hemoglobin levels were measured using a hemoglobin assay kit (Chondrex Inc., Woodinville, WA), which correlates with a cyan...
In addition to annexin-V staining, caspase 3 and 8 activities were quantified under the same treatment regime using the same number of cells according to instructions of the colorimetric assay kits (BD Bioscience Clontech). Briefly, cell pellets collected from each well were lysed in 50 μ...
(brown fits,Fig. 2b; and brown histogram,Fig. 2d). The absence of the∼6nm intermediate in the AzuC112Acase firmly suggests that the∼6nm intermediate observed in the unfolding trajectories of wildtype (wt)-Azu (Fig. 1d) is indeed triggered by the mechanical disruption of the Cu–S...
HA Ravin An improved colorimetric enzymatic assay of ceruloplasmin J Lab Clin Med, 58 (1961), pp. 161-168 View in ScopusGoogle Scholar 21 KH Schosinsky, HP Lehmann, MF Beeler Automated determination of serum ceruloplasmin activity with o-dianisidine dihydrochloride as substrate Clin Chem, 21 (19...
Cell viability was evaluated using colorimetric MTS assay that measures cellular metabolic activity. Cells were seeded into 96-well plates at 5 × 103 cells per well in 80 μl full medium for 24 h, after that Complex (50 µM), Ligand (50 µM and 100 µM), DMSO ...
Lipid peroxidation was analyzed using a Lipid Peroxidation Colorimetric/Fluorometric Assay Kit (BioVision, UK) by means of malondialdehyde (MDA) content. Human neutrophils were seeded in a 96-well plate at a density of 20 × 104neutrophils per well (99 μL aliquots/well) and exposed to 1 μ...