The residue is dissolved in hexane, the antibiotic is extracted with 75% methanol in water, and the extract is diluted to the assay range with a final methanol concentration of 25%. Microbiological activity by horizontal agar diffusion is measured by the cylinder plate technique with Bacillus ...
IN the course of experiments designed to study cytochemical changes which occur in susceptible cells under the influence of penicillin, we applied various stains and reagents to agar plates prepared as for the standard F D A cylinder-plate assay, which is essentially similar to that recommended by...
Clavulanic acid is a natural occurring beta-lactam product of Streptomyces clavuligerus and is a potent inhibitor of bacterial beta-lactamases. The present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibitory...
SUMMARY: The use of plate glass sheets carrying twenty-four cylinders in place of Petri dishes for the biological assay of streptomycin by the cylinder-plate method initially led to large assay errors. The substitution of cavities for cylinders, of a single layer of agar for two layers, one ...
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A revision is proposed of the cylinder-plate assay for penicillin in which two concentrations of an unknown preparation are compared with two equivalent concentrations of the working standard. A complete assay consists of three four-plate groups, each testing a separately weighed sample of the dry ...