CycloheximideChaseAnalysisofProteinDegradationinSaccharomyces cerevisiae BryceW.Buchanan 1 ,MichaelE.Lloyd 1,2 ,SarahM.Engle 1 ,EricM.Rubenstein 1 1 DepartmentofBiology,BallStateUniversity 2 BioproductResearch&Development,EliLillyandCompany Correspondenceto:EricM.Rubensteinatemrubenstein@bsu.edu URL:http://...
Cumulative fatigue damage and life prediction theories--a survey of the state of the art for homogeneous materials 热度: 100 Simple Secrets of the Best Half of Life 热度: FigureS1.Cycloheximide(CHX)chaseassayand[ 35 S]methioninepulse-chaseassayfor ...
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
3.2Cycloheximide chase assay The puncta-counting assay provides a means of testing whether the clearance of transient inclusions (e.g., Q-bodies, JUNQ, and/or INQ) that contain misfolded proteins is impaired in differentgeneticbackgrounds. However, it is possible that some genetic backgrounds cause...
Twitter Google Share on Facebook cycloheximide Acronyms Encyclopedia Wikipedia cy·clo·hex·i·mide (sī′klō-hĕk′sə-mīd′, -mĭd) n. A colorless crystalline compound, C15H23NO4, that is used as an agricultural fungicide. American Heritage® Dictionary of the English Language, Fifth...
Cycloheximide（放线菌酮）(Abmole, M4879，纯度>99%) 是一种蛋白生物合成抑制剂, IC50值为532.5 nM。作者在研究过程中，采用放线菌酮(CHX) chase法检测RAB1A的半衰期。简而言之，细胞与蛋白质合成抑制剂CHX (100 μg/ml)处理不同的时间。然后提取处理后的细胞蛋白，进行Western blotting检测RAB1A的表达。通过...
Behavior Of SlGFP Puncta In Cycloheximide Chase Experiments.Daniel W. SummersKatie J. WolfeHong Yu RenDouglas M. Cyr
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Buchanan BW, Lloyd ME, Engle SM, and Rubenstein EM (2016) Cycloheximide chase analysis of protein degradation in Saccharomyces cerevisiae. J. Vis. Exp. (110), e53975 Cycloheximide Solution from TOKU-E: Jimenez-Moreno N et al (2019) LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human...