Thus, the CHX chase assay is widely recognized and used to observe intracellular protein degradation and to determine the half-life of a given protein in eukaryotes. Here, we present a complete experimental procedure of the CHX chase assay. Graphical overview...
the level of remaining Drp1 at different time points was quantified as the percentage of initial Drp1 level (0 hour of CHX treatment). C and D, the half-life of Drp1 in the presence of GFP-Parkin or GFP alone was analyzed by [35S] methionine pulse-chase assay, and the protein levels ...
Cumulative fatigue damage and life prediction theories--a survey of the state of the art for homogeneous materials 热度: 100 Simple Secrets of the Best Half of Life 热度: FigureS1.Cycloheximide(CHX)chaseassayand[ 35 S]methioninepulse-chaseassayfor ...
Cycloheximide (CHX) chase assay and [35S] methionine pulse-chase assay for the half-life of Drp1. A, 293T cells were transfected with GFP-Parkin and GFP-Parkin (ΔR2) for 24 hours. Cells were then treated with CHX (100 μg/ml) for the indicated hours, and Western blotting was ...
翻译 cycloheximidechaseassay 翻译 cycloheximidechaseassay 以上结果来自机器翻译。 释义
Define cycloheximide. cycloheximide synonyms, cycloheximide pronunciation, cycloheximide translation, English dictionary definition of cycloheximide. n. A colorless crystalline compound, C15H23NO4, that is used as an agricultural fungicide. American Heri
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After a 3 h chase, the EGFR is still accumulated at a perinuclear endosomal compartment in control cells as well as in cells expressing GFP–MICAL-L1. In contrast, MICAL-L1-depleted cells exhibited different EGFR distribution. EGFR vesicles are not detected at the perinuclear region but are ...
| cellresearchcorp | cellsciences | cellscript | cellsignal | celltechnology | Celltreat Scientific Products | cellular technology ltd | cellutions biosystems | cellworks | celsis | cemines | cerilliant | ch3 biosystems | chantest | charles river laboratories | Chase Instruments | Chemetrics Inc...