These data indicate that antibody-based neutralization of CXCL16 limits the generation of forebrain CD8+CD103+ T cells and suggests CXCL16 might be a therapeutic target. Fig. 7 CXCL16 neutralization leads to decreased percentage of TRM cells in the CNS after viral clearance. a. Schematic ...
Furthermore, anti‐CXCL16 monoclonal antibody significantly reduced the clinical arthritis score and reduced infiltration of inflammatory cells and bone destruction in the synovium of mice with CIA. Conclusion Our results indicate that CXCL16 plays an important role in T cell accumulation and stimulation...
The effect of anti-CXCL16 monoclonal antibody on murine collagen-induced arthritis (CIA) was evaluated.ResultsCXCL16 was expressed in RA synovium. CXCR6 was expressed more frequently on synovial T cells than in peripheral blood. Moreover, CXCR6-positive synovial T cells more frequently expressed ...
This antibody has also been reported to neutralize the bioactivity of ovine IL-8, however prior removal of sodium azide may be necessary for functional assays. Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to...
This induction of PI3K activity could also be blocked specifically by pretreating the cells with antibody to IL-18 before the addition of IL-18 (data not shown). Akt is one of the downstream substrates for PI3K. Western blot analysis using anti-phospho Akt (Thr308) antibodies, which ...
This antibody has also been reported to neutralize the bioactivity of ovine IL-8, however prior removal of sodium azide may be necessary for functional assays. Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to...
N-terminal CXCL16 and ADAM-10 were significantly higher in stage-III than stage-II, but no significant difference was observed in the C-terminal fragment of CXCL16. Further, stimulation of the CXCR6/CXCL16 axis activated Src, FAK, ERK1/2, and PI3K signaling pathways, as per antibody micro...
Receptor surface expression was verified by flow cytometry using hACKR2-specific mAb (clone 196124, R&D Systems, Minneapolis, MI, USA) or polyclonal mACKR2-specific antibody (ab1656, Abcam, Cambridge, UK). The absence of CXCR3 at the cell surface was confirmed using mAb clone 1C6 and the ...