Cx3cr1-GFP小鼠是一种在Cx3cr1基因座插入绿色荧光蛋白(GFP)基因的转基因小鼠模型。Cx3cr1(趋化因子受体3,与趋化因子CX3CL1配体结合)主要在单核细胞、巨噬细胞、微小胶质细胞和某些树突状细胞中表达。Cx3cr1-GFP小鼠主要用于研究这些细胞的分布、功能及其在免疫反应和神经系统中的作用。 Cx3cr1的功能: 细胞...
Cx3cr1-GFP and Ccr2-RFP dual positive activated microglia were observed in the outer retina and subretinal space of 6- and 8-week-old animals. Ccr2-RFP single positive bone marrow-derived macrophages were observed to migrate into the retina of Mertk (-/-) Cx3cr1 (GFP/+) Ccr2 (RFP/...
This protocol provides a detailed approach for generation of retinal B-scans, with SD-OCT, and imaging of microglia cell distribution in Cx3cr1 gfp/gfp mice with SLO in vivo, using an ophthalmic imaging platform system. The protocol can be used in several reporter mouse lines. However, ...
Using CX3CR1-/- mice, in which the CX3CR1 gene is substituted with the gene for the GFP on both alleles (CX3CR1GFP/GFP), and a murine model of transient brain ischemia, we find that CX3CR1 deficiency resulted in a decrease in the size of ischemic lesion, a decrease in the ...
Because protein expression may not always coincide with mRNA transcription, a subset of mice were injected with saline or LPS and CD14 protein expression was determined on GFP+/CD45low microglia 24 h later. Representative histograms of CD14 staining of microglia are shown in Figure 4F. LPS ...
5 reagents referenced in Analyses of microglia effector function using CX3CR1-GFP knock-in mice.
(C-X3-C motif) ligand 1; CX3CR1: CX3C chemokine receptor 1; CX3CR1−/−: CX3CR1-deficient; ELISA: enzyme-linked immunosorbent assay; FACS: Fluorescence Activated Cell Sorter; GFP: green fluorescent protein; IL: interleukin; MCAO: middle cerebral artery occlusion; MRI: magnetic ...
(f) Absolute number of CD11b+ CX3CR1+ cells in the blood and lung parenchyma of CX3CR1gfp/gfp mice after 1 month BCG vaccination (n = 3-5). (g) BCG CFUs in antibiotic-treated and BCG-vaccinated mice (n = 5). Data are presented as mean ± s.e.m with * p <...
在HexbtdT/tdT小鼠的生理状态下,免疫荧光染色及3D重构结果显示几乎所有CNS不同区域的Iba1+小胶质细胞都被标记成红色,且在非Iba1+细胞中没有检测到任何异位tdT表达(图一b-d)。相比之下,Cx3cr1GFP/+小鼠的GFP+细胞不仅标记了小胶质细胞,还标记了血管周围巨噬细胞(pvMΦ)和软脑膜下巨噬细胞(mMΦ)(图一e)。
Studies of Cx3cr1gfp/gfp knock-in mice, in which the Cx3cr1 gene has been replaced by Gfp, have revealed that CX3CR1-deficient microglia show excessive cellular activation and overproduction of inflammatory mediators, generally increasing susceptibility to CNS inflammatory diseases17,23. However,...