Fig2. Cx3cr1EGFP/+mice Cortex EGFP positive cells co-localize with IBA1. 相关品系 Cx3cr1-Flox 品系名:C57BL/6Smoc-Cx3cr1em1(flox)Smoc 品系状态:活体 | 目录号:NM-CKO-226496 Cx3cr1-CreERT2 品系名:C57BL/6Smoc-Cx3cr1em1(creERT2-WPRE-polyA)Smoc ...
2016. Evaluation of TgH(CX3CR1-EGFP) miceimplanted with mCherry-GL261 cells as anin vivo model for morphometrical analysisof glioma-microglia interaction. BMC Cancer:1-13.Resende, F. F., Bai, X., Del Bel, E. A., Kirchhoff, F., Scheller, A., and Titze- de-Almeida, R. (2016)....
In these mice, the first 390 base-pairs of the coding exon 2 of the Cx3cr1 gene are replaced by the enhanced green fluorescent protein (EGFP) sequence, thereby resulting in a loss of Cx3cr1 gene expression. Trem2−/− mice on the C57BL/6 J background were purchased through ...
An EGFP cassette was inserted in place of the first 390 bp of the second exon of the gene for CX3CR1 that disrupts the CX3CR1 gene and labels all cells that would normally express CX3CR1 with a green fluorescent protein [32]. Mice were housed in polypropylene cages and maintained at...
Mice C57Bl6 mice were purchased from Elevage Janvier (Le Genest, Saint Isle, France). Cx3cr1-EGFP-Kin (Cx3cr1gfp/+),17 Csf1r-Gal4VP16/UAS-ECFP (MacBlue),18 and Ccr2−/− mice were intercrossed to generate MacBlue×Cx3cr1gfp/+, MacBlue×Cx3cr1gfp/gfp, or MacBlue×Cx3cr...
Rag1-/- and Rag1-/-Cx3cr1-eGFP/eGFP mice were born and raised under germ-free conditions in sterile isolators, at the University of Bern animal facility. At the age of 7 weeks, mice were co-housed for 4 weeks with a female mouse colonized with the sDMDMm2 gnotobiotic microbiota consi...
we examined whether the lack of functional CX3CR1 in homozygous mice (CX3CR1EGFP/EGFP, KO) alters the ability of MPs to initiate immune responses during epididymitis induced by LPS intravasal–epididymal injection. Confocal microscopy revealed that CX3CR1-deficient MPs located in the initial se...
结论:本课题成功构建慢病毒过表达载体pUbi—MSC—CX3CRl一EGFP,及滴度为2x108TU/ml的,LV-CX3CRl一EGFP慢病毒液。为下一步研究慢病毒介导的CX3CRl基因转染骨髓间充质干细胞及其归巢能力的作用奠定基础。关键词:CX3CR.1:趋化因子;慢病毒载体;’ 重庆医科大学硕士研究生学位论文THECONSTRUTIONANDIDENTIFICATIONOFMI...
to 14-day-old wild-type C57BL/6J mice and CAG-EGFP mice. Adipose tissues were digested incubated with 2 mg/mL collagenase II (Sigma-Aldrich, USA) and were separately resuspended in DMEM/F-12 complete medium. Unless explicitly stated otherwise, all experiments used EGFP-ADSCs for easy ...
However, the rod and cone ERG oscillatory potentials were significantly reduced in the CDKO animals, a phenotype apparent in Cx3cr1(EGFP/EGFP)- but not Ccl2(-/-)-founder lines. This correlated with aberrant amacrine cell morphology in the CDKO mice. In addition, Müller cells were gliotic...