In Cx3cr1CreER-Eyfp/wt mice, the protein-coding exon of one of the Cx3cr1 alleles is replaced by the Cre-ERT2 fusion gene and the enhanced yellow fluorescent protein gene (Eyfp), implying that all CX3CR1+ cells (mainly microglia in the CNS parenchyma) should express both Cre-ERT2 a...
CreERT2通常定位于细胞质中,只有在他莫昔芬(TAM)诱导后CreERT2才会进入细胞核,从而识别loxP位点发生基因重组。因此HexbCreERT2小鼠可用于小胶质细胞的诱导型功能研究。将HexbCreERT2小鼠与报告基因小鼠R26yfp/yfp进行交配,在阳性子代出生后第一天和第三天(P1和P3)分别注射TAM,P42小鼠经检测发现仅有小胶质细胞表达Y...
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