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CRLs are protein complexes that include four subunits: a RING H2 finger protein (e.g. Rbx1/Roc1), a member of the cullin family proteins (e.g. Cul1), an adaptor protein (e.g. Skp1) and a substrate recognition subunit (e.g. F-box) [73]. Ubiquitinylation of most of the NF-κB...
为解析IP6如何同时招募Cul4/Rbx1, 研究团队利用所解析的高分辨率晶体结构对CRL4-CSN复合物的冷冻电镜(Cryo-EM)结构进行装配,意外的发现了和IP6完美匹配的电子云,并鉴定出Rbx1上的K25/26残基和IP6直接互作。鉴于IP6的结合口袋由CSN2和Rbx1组成,Cul4不直接参与,研究团队提出IP6是广谱的CRL-CSN分子间胶水的假设,...
TIP120A formed a complex with CUL1 and Rbx1, but interfered with the binding of Skp1 and F-box proteins to CUL1. TIP120A greatly reduced the ubiquitination of phosphorylated IκBα by SCFβ-TrCP ubiquitin ligase. These results suggest that TIP120A functions as a negative regulator of SCF ...
such as leucine-rich, WD repeat and ankyrin repeats, which mediate interactions with their substrate-specific interaction motif. Genome mining inPlasmodiumidentified SKP1, Cullin1, and RBX1 together with two F-box domain-containing proteins19. However, their roles during thePlasmodiumdevelopment remain...
Arabidopsis Has Two Redundant Cullin3 Proteins That Are Essential for Embryo Development and That Interact with RBX1 and BTB Proteins to Form Multisubunit ... Cullin-based E3 ubiquitin ligases play important roles in the regulation of diverse developmental processes and environmental responses in eukaryo...
Here, using highly quantitative mass spectrometry, we demonstrate that this problem is addressed by CAND1, a factor that controls the dynamics of the global cullin 1-RING ubiquitin ligase network by promoting the assembly of newly synthesized F-box containing substrate receptors with CUL1-RBX1 core...
SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the den...
1 Cullin3分子的结构 CRLs家族成员以蛋白复合体的形式存在,包括小RING蛋白(ROC1,ROC2)、Cullin蛋白、linker(如Skp1,Elongin B/C,DDB1等)和底物识别亚基(如F-box,DCAF)。Cullin蛋白发挥支架的作用,其N端结构域结合接头蛋白和底物蛋白识别区域结合募集底物分子,C端结构域则结合RING蛋白(Rbx1或Rbx2),招募E2泛素结...
. The CUL1 C-terminus binds Rbx1 and the N-terminus binds the adaptor Skp1. Rbx1 facilitates the recruitment of the E2 to the complex [13]. The adaptor Skp1 binds the SRS, which is an F-box protein that links to Skp1 through the F-box motif. The F-box protein binds and positions...