The CUL4-DDB1 ubiquitin ligase regulates cell proliferation, survival, DNA repair, and genomic integrity through targeted ubiquitination of key regulators, yet the substrate receptors that dictate the specificity of this ubiquitination machinery have been largely unknown. Recent work identified a family ...
mechanistic target of rapamycin kinase complex 1; NAE1, NEDD8 activating enzyme E1 subunit 1; NOC, nocodazole; RING, really interesting new gene; RBX1, ring-box 1; SA-GLB1/尾-gal, senescence-associated galactosidase beta 1; TSC2, TSC complex subunit 2; TUBA, tubulin alpha; WIPI2, WD ...
et al. Arabidopsis has two redundant Cullin3 proteins that are essential for embryo development and that interact with RBX1 and BTB proteins to form multisubunit E3 ubiquitin ligase complexes in vivo. Plant Cell 17, 1180–1195 (2005). 46. Spoel, S. H. et al. Proteasome-mediated turnover ...
CDT1, a licensing factor in pre-RC, is targeted by CRL4 (DDB1-CUL4-RBX1 Cullin-RING ubiquitin Ligase 4) during the transition between the G1 and S phases of the cell cycle, and by CRL1 (SKP1-CUL1-F-box, or SCF) during S and G2 phase13,14,15,16. In most cells, SCF ...
所有的Cullin通过C-端区域与RING finger protein Rbx1/Hrt1相互作用,然后招募E2泛素结合酶。Cullin的N-端结构域分别与不同种类的底物特异的接头蛋白(adaptor)相互作用。人基因组表达两种Cul4的同源蛋白:Cul4A蛋白和Cul4B蛋白,它们通过N-端区域与Ddb1蛋白相互作用,然后Ddb1蛋白与各种底物特异的接头蛋白相互作用。这些...