coli were multidrug resistant and ESBLs producer. While all isolates were sensitive to Imipenem and Amikacin. PCR technique was performed to detect ESBLs blaCTX-M gene, the results revealed that (100%) of E.coli isolates carried this gene....
圈直径 之差≥5 rnlTl可判定为 ESBLs 阳性 菌株 。 1.2.2 细 菌质粒 DNA 的提取 根据质粒提取试剂盒说明书提取细菌质粒 DNA ,一20℃ 保存备用。 1.2.3 TEM、SHV 和 CT x _M 基因 PCR 片段 引物设计 TEM、SHV 和 CTx_M基因序列查 自GenBank,登录号分 ...
CTX—M encodinggene and study the epidemiologicalaspect of CTX-M-producing isolates. MethodsAtotalof254clinicalisolates ofmultidrug resistant gram—negative bacilliwerecollectedfromOctober2004to July 2005. ESBL—producing isolateswereconfirmed by means ofphenotypic confirmatory testsasrecommended by theCLSI/NCC...
目的:探讨来源于呼吸道肺炎克雷伯菌(KP)耐药性与CTX-M型超广谱β-内酰胺酶(ESBLs)的关系.方法采用PCR 扩增和测序分析检测 KP 质粒和染色体上的 CTX-M 型 ESBLs 基因;采用纸片扩散法检测抗菌药物敏感性;使用χ2检验分析相关数据.结果有 CTX-M-1群酶和 CTX-M-25群酶检出,CTX-M 型 ESBLs 的总检出率为...
据有关报道,CTX-M型和TEM型或SHV型的同源性较低,仅为40%[2]。目前发现的的CTX-M型ESBLs种类超过107种,根据其氨基酸序列的差异(大于94%的相同序列认为是一个群,小于90%的相同序列认为是不同的群)分为5个群[3]:CTX-M-1 群包括 CTX-M-1、CTX-M-3、CTX-M-10、CTX-M-12、CTX-M-15、FEC-1、...
[ 结论] CTX—M 一15基因是大肠埃希菌和肺炎克雷伯菌对头孢他啶耐药的主要机制,建议临床合理使用抗生素,避免引起流行。关键词:CTX—M 一15;头孢他啶耐药;大肠埃希菌;肺炎克雷伯茵中图分类号:R378.21文献标志码:A文章编号:1671—7295( 2012) 05—0495—04CTX—M 一15genedetected i n ceftazi di m e—...
【摘要】为了探讨凉粉草含药血清与抗菌药联合使用对连续传代的携带fosA3耐药基因的产CTX-M型ESBLs大肠埃希菌的体外抑菌活性,应用二倍微量稀释法测定各抗菌药及凉粉草含药血清对细菌的最小抑菌浓度(MIC)值,以凉粉草含药血清的亚抑菌浓度(1/2 MIC)与抗菌药联合使用对大肠埃希菌进行传代.结果发现,凉粉草含药血清联...
1.METHODS The antimicrobial susceptibility test on ESBLs KPN which we have known its phenotype was conducted, then the CTX-M gene was amplified by polymerase chain reaction and detected.结果14株产ESBLs肺炎克雷伯菌药敏结果一致,耐三代头孢和耐酶抑制剂,均检出CTX-M基因。 2.Methods The CTX-M ge...
对头孢噻肟、氨曲南、庆大霉素等的耐药率均〉50.0%;56株多药耐药大肠埃希菌中,44株检出CTXM-14酶基因,28株检出CTX-M-15酶基因,检出率分别为78.6%和50.0%;携带CTX-M-14与CTX-M-15酶基因的多药耐药大肠埃希菌对头孢噻肟、头孢他啶的耐药率分别81.8%、52.2%和96.4%、60.7%,两者相比较,差异有统计学意义(...
The 17 isolates were shown to have blaCTX-M genes that were associated with large plasmids (≥58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from...