Proteins were ethanol/ace- tone precipitated, re-dissolved in 7M urea, 2 M thiourea, 20 mM dithiothreitol (DTT), and the protein amount was estimated using Qubit Protein Assay (Thermo Fisher Scientific). Following alkylation, pH of the samples was adjusted to 8 and proteins were digested...
In this study, we measured cerebrospinal fluid (CSF) levels of amyloid-andprotein in MS and in clinically isolated syndrome patients, as both proteins have been associated with cognitive decline in Alzheimer's disease (AD). In AD, amyloid-accumulates in the brain as insoluble extracellular ...
Proteins were ethanol/ace- tone precipitated, re-dissolved in 7M urea, 2 M thiourea, 20 mM dithiothreitol (DTT), and the protein amount was estimated using Qubit Protein Assay (Thermo Fisher Scientific). Following alkylation, pH of the samples was adjusted to 8 and proteins were digested...
The presence of intrathecal synthesis of immunoglobulins was one of the bases for testing anti-B-cell therapies, such as anti-CD20 monoclonal antibodies, which proved to be among the most effective approved therapies in MS. [34]. Interestingly, however, evidence supports that the role of B cell...
protein. ELISA experiments allowed validating part of the results obtained in the proteomics analysis and showed that some of the alterations in the CSF proteome are also mirrored in serum samples from multiple sclerosis patients. CSF of multiple sclerosis patients was characterized by TTR ...
Using the Protein-A plaque assay, numbers of IgG + IgA + IgM producing cells determined in patients with multiple sclerosis (MS) were 0.1–5% in CSF and 0.1–0.7% in peripheral blood; interestingly, 7 of 11 MS patients had IgM producing cells in CSF. In patients with aseptic meningitis ...
Label free relative protein quantification was performed based on peptide spec- tral counts (SCs) and the extracted ion current mea- surements. Mann Whitney U-tests were used to compare FH and C3 levels between MCI-AD and mild AD (n = 16) due to the small sample size. Statistical ...
[62]M. Colucci, L. Roccatagliata, E. Capello et al., “The 14-3-3 protein in multiple sclerosis: a marker of disease severity,” Multiple Sclerosis, vol. 10, no. 5, pp. 477–481, 2004. [63]F. G. Joseph, C. L. Hirst, T. P. Pickersgill, Y. Ben-Shlomo, N. P. Robertson...
The two chemokines, monocyte chemoattractant protein (MCP)-1 and γ-interferon inducible protein (IP)-10, are thought to be involved in the pathogenesis of multiple sclerosis (MS). We measured MCP-1 and IP-10 levels in serum and CSF samples from 38 acute and 25 stable MS patients and fro...
The present paper is concerned with a correlation between clinical parameters of multiple sclerosis (MS) and different agar gel electrophoresis patterns in cerebrospinal fluid (CSF). Agar gel electrophoresis clearly represents the most sensitive method f