Cryptococcus neoformansPolysaccharidesMagnetic Resonance SpectroscopyMolecular ConformationMannansSonicationA series of polysaccharides was derived by physical and chemical methods from an antigenic, O-acetyl-containing, glucuronoxylomannan (GXM), isolated from the growth medium of Cryptococcus neoformans (CDC B...
Cryptococcus neoformans cells have very large capsules (FIG. 1), which are composed of the polysaccharide, glucuronoxylomannan (GXM). Serotype A Go has an α-1,3-D-mannose backbone with one β-D-glucuronide and two β-D-xyloside sugars per each trimer of mannose (FIG. 2) (14). The...
We synthesized Cryptococcus neoformans serotype A glucuronoxylomannan (GXM) conjugate vaccines under conditions suitable for human use to prevent disseminated cryptococcosis. The purified, sonicated GXM was derivatized with adipic acid dihydrazide through either hydroxyl or carboxyl groups and then covalently...
In this work, we evaluated the immunomodulatory role of the capsular polysaccharides GXM and GalXM fromC. neoformansin dendritic cells that are, together with alveolar macrophages, important in regulating the innate immune response against lung infection byC. neoformans61,62,63,64. Initial results us...
Therefore we will investigate the possibilities to use GXM and MP4 therapeutically. 展开 关键词: Cryptococcus neoformans epidemiology HIV HAART surfactant proteins cytokines chemokines leucocyte-migration capsular polysaccharides 出版时间: 1998/09/17
neoformans is an extensive polysaccharide capsule which is composed of two polymers, glucuronoxylomannan (GXM) and galactoxylomannan (GalXM). The structures of these polysaccharides have been chemically defined, but their biosynthesis is not understood. The proposed work addresses this gap in ...
neoformans with epithelial lung cells, in the presence or absence of capsule components. Two fungal strains, the wild type, NE-241, and a mutant, CAP67, deficient in GXM production, were used throughout this study. The adhesion assays were completed using epithelial lung cells, A549, and ...
The Cryptococcus neoformans capsular polysaccharide glucuronoxylomannan (GXM) has been conjugated to tetanus toxoid (GXM-TT) as an investigational vaccine... L Pirofski,R Lui,M Deshaw,... - 《Infection & Immunity》 被引量: 137发表: 1995年 A proteomic‐based approach for the identification of...
neoformans GXM promoted phagocytosis by J774.16 macrophage-like cells in media that contained heat-inactivated FCS (Mukherjee et al., 1995b). This suggested a C-independent process, but the presence of heat-inactivated FCS in the media raised the possibility that another serum constituent mediated ...
Monoclonal antibodies (MAbs) reactive with glucuronoxylomannan (GXM), the major capsular polysaccharide of the yeast Cryptococcus neoformans, produce distinct capsular reactions when viewed by differential interference contrast microscopy. These reactions depend on the epitope specificity of the antibody. Op...