This review briefly explains several key bottlenecks for atomic resolution CryoEM, and describes the corresponding solutions for these bottlenecks based on the recent technical advancements. The review also aims to provide an overview about the technical differences between its applications in biology and...
The membrane attack complex (MAC) is one of the immune system’s first responders. Complement proteins assemble on target membranes to form pores that lyse pathogens and impact tissue homeostasis of self-cells. How MAC disrupts the membrane barrier remai
Abstract Main Results Discussion Methods Data availability Code availability References Acknowledgements Author information Ethics declarations Peer review Additional information Extended data Supplementary information Source data Rights and permissions About this article This article is cited by AdvertisementNature...
Purpose of Review We discuss recent advancements in structural biology methods for investigating sites of protein-protein interactions. We will inform read... R Carter,A Luchini,L Liotta,... - 《Current Pathobiology Reports》 被引量: 0发表: 2019年 Correlative Fluorescence and Electron Microscopy Th...
@therickybaker Peer review should include raw data and full processing directories submitted to EMPIAR. @IsraelF961350888 hours agoThe his is a great place/lab/boss to do science! Apply! https://t.co/uv7I0l1lUf @unitmporg14 hours agoDisulfide constrained Fabs overcome target size ...
Here, we review techniques for map interpretation and provide examples from recent studies of multi-protein complexes. Copyright 2019 MRC Laboratory of Molecular Biology. Published by Elsevier Ltd.. All rights reserved. 展开 DOI: 10.1016/j.sbi.2019.06.009 年份: 2019 ...
This article though not a complete review of PX or SPCryoEMof macromolecular protein complexes, serves as a guideline in understanding the underlying principles. 展开 关键词: Protein crystallography single particle cryo electron microscopy hybrid methods protein structure macromolecular complexes image ...
2.5 Current strategies for optimizing preparation of cryo-grids. Air-water interface and physics of thin films. How to troubleshoot. Review of different freezing hardware devices. 3 Data collection 3.2 Radiation damage in cryo-EM. Sources of radiation damage, role of electron energy, exposure weigh...
Advanced Review, May 2021. [paper] 2.4. Cryo-EM Map Sharpening Methods CryoTEN: Efficiently Enhancing Cryo-EM Density Maps Using Transformers Joel Selvaraj, Liguo Wang, Jianlin Cheng bioRXiv, September 2024. [paper][code][CryoTEN] Integrating AlphaFold and deep learning for atomistic interpretation...
Peer Review File Description of Additional Supplementary Files Supplementary Movie 1 Supplementary Movie 2 Reporting Summary Rights and permissions Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and repro...