Giulia Weissenberger1,2,3, Rene J. M. Henderikx1,2,3 and Peter J. Peters 2 ✉ 低温电子显微镜(cryo-EM)迅速成为结构生物学领域中一种有吸引力的方法。 随着cryo-EM的爆炸式增长,必须改进样品前处理以防止工作流程中的拥塞。 对改进的显微镜样品的迫切需求导致了方法的多样化。 这篇综述旨在...
24. Clapham, D. E. TRP channels as cellular sensors.Nature426, 517 (2003). 25. Moran, M. M. TRP channels as potential drug targets.Annual review of pharmacology and toxicology58, 309-330 (2018). 26. Hellmich, U. A. & Gaudet, R. Structural Biology of TRP Channels. (Springer Intern...
In this review, recent advances in the structural understanding of virus-antibody interactions are outlined for whole virions with icosahedral T = pseudo 3(picornaviruses) and T = 3(flaviviruses) architectures, focusing on the dynamic nature of viral shells in different functional states. Glycoprotein...
sample preparation must evolve to prevent congestion in the workflow. The dire need for improved microscopy samples has led to a diversification of methods. This Review aims to categorize and explain the principles behind various techniques in the preparation of vitrified samples for the electron micro...
Review Article Published: 08 June 2018 Cryo-EM in drug discovery: achievements, limitations and prospectsJean-Paul Renaud, Ashwin Chari, Claudio Ciferri, Wen-ti Liu, Hervé-William Rémigy, Holger Stark & Christian Wiesmann Nature Reviews Drug Discovery volume 17, pages 471–492 (2018)...
传统EM又脱水又真空,而你看这个可以不脱水不破坏蛋白质结构进行EM,多好。拍几张照片就可以统计3D建模,简直是结构生物学的地图炮,学术灌水的消防栓啊。有兴趣可以读读下面这篇review。Cryo-electron microscopy<img src="http://t.cn/A6GSQg7D" data-rawwidth="597" data-rawheight="475" class="origin_image...
In this review, we illustrate the contributions of cryo-EM and related methods to the structure determination of amyloid fibrils extracted post mortem from patient brains or formed in vitro. We also discuss cell models of protein aggregation and the contributions of electron tomography to ...
ex vivo prion fibrils from hamster 263K and mouse RML prion strains were reported. The fibrils have a comparable parallel in-register intermolecular β-sheet (PIRIBS) architecture that now provides a structural foundation for understanding prion strain diversity in mammals. Here, we review these new...
Thanks to the cryo-EM structures presented in this review, we are now beginning to understand how TA proteins are selectively engaged, how energetic barriers for membrane translocation are lowered and which conformational changes promote biogenesis. In the future, integration of single-particle cryo-...
This review provides breakthroughs and achievements in the ... H Yao,X Wang,J Chi,... - 《Molecules》 被引量: 0发表: 2024年 Cryo-EM studies of membrane proteins at 200keV proteins and the impact of cryo-EM, and review the technical and practical benefits, achievements and limitations of...