In this review, we observe membrane protein structures from the Protein DataBank and their associated maps in the Electron Microscopy DataBase and determine how the SP-TEM maps allow lipid visualization, the typ
低温电子显微镜(cryo-EM)迅速成为结构生物学领域中一种有吸引力的方法。 随着cryo-EM的爆炸式增长,必须改进样品前处理以防止工作流程中的拥塞。 对改进的显微镜样品的迫切需求导致了方法的多样化。 这篇综述旨在对电子显微镜玻璃化样品的制备中各种技术背后的原理进行分类和解释。 讨论了工作流程中的各个方面和挑战,从...
Review Article Published: 08 June 2018 Cryo-EM in drug discovery: achievements, limitations and prospectsJean-Paul Renaud, Ashwin Chari, Claudio Ciferri, Wen-ti Liu, Hervé-William Rémigy, Holger Stark & Christian Wiesmann Nature Reviews Drug Discovery volume 17, pages 471–492 (2018)...
Annual review of pharmacology and toxicology 58, 309-330 (2018). 26. Hellmich, U. A. & Gaudet, R. Structural Biology of TRP Channels. (Springer International Publishing, 2014). 27. Khoshouei, M., Danev, R., Plitzko, J. M. & Baumeister, W. Revisiting the structure of haemoglobin ...
Modeling flexible macromolecules is one of the foremost challenges in single-particle cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental questions in structural biology. We introduce Three-Dimensional Flexible Refinemen
Software updates Release management Thermo Fisher Scientific offers a constant stream of software improvements and updates, ensuring you can achieve your goals as quickly and easily as possible. Learn more Cookie设置 我们及我们的关联方等通过运用Cookie等技术来为您提供您感兴趣的网站定制内容、识别访问者、...
In this webinar, we review the capabilities that have recently been added to Smart EPU Software, and how they can further streamline single particle analysis workflows. What’s new in Smart EPU Software? Smart screening: New AI-driven session type captures atlases, automates grid square and foil...
An exhaustive review of DNA repair pathways is beyond the scope of this work, and we recommend other recent publications [1–3,8,19,20]. The HR pathway is intrinsically complex and involves a multitude of proteins and several perfectly synchronized stages (Figure 1 and Table 1). Show ...
ex vivo prion fibrils from hamster 263K and mouse RML prion strains were reported. The fibrils have a comparable parallel in-register intermolecular β-sheet (PIRIBS) architecture that now provides a structural foundation for understanding prion strain diversity in mammals. Here, we review these new...
Particle picking is a time-consuming step in single-particle analysis and often requires significant interventions from users, which has become a bottleneck for future automated electron cryo-microscopy (cryo-EM). Here we report a deep learning framework, called DeepPicker, to address this problem ...