Giulia Weissenberger1,2,3, Rene J. M. Henderikx1,2,3 and Peter J. Peters 2 ✉ 低温电子显微镜(cryo-EM)迅速成为结构生物学领域中一种有吸引力的方法。 随着cryo-EM的爆炸式增长,必须改进样品前处理以防止工作流程中的拥塞。 对改进的显微镜样品的迫切需求导致了方法的多样化。 这篇综述旨在...
24. Clapham, D. E. TRP channels as cellular sensors.Nature426, 517 (2003). 25. Moran, M. M. TRP channels as potential drug targets.Annual review of pharmacology and toxicology58, 309-330 (2018). 26. Hellmich, U. A. & Gaudet, R. Structural Biology of TRP Channels. (Springer Intern...
In this webinar, we review the capabilities that have recently been added to Smart EPU Software, and how they can further streamline single particle analysis workflows. What’s new in Smart EPU Software? This tutorial introduces two new features now available in Smart EPU Software: A targeting ...
In this webinar, we review the capabilities that have recently been added to Smart EPU Software, and how they can further streamline single particle analysis workflows. What’s new in Smart EPU Software? This tutorial introduces two new features now available in Smart EPU Software: A targeting ...
This review critically examines the evolution and current state of cryo-EM particle picking methods, with an emphasis on the impact of AI. We conducted a comparative evaluation of popular AI-based particle picking methods, using both general machine learning metrics and specific cryo-EM structure ...
sample preparation must evolve to prevent congestion in the workflow. The dire need for improved microscopy samples has led to a diversification of methods. This Review aims to categorize and explain the principles behind various techniques in the preparation of vitrified samples for the electron micro...
In this review, we observe membrane protein structures from the Protein DataBank and their associated maps in the Electron Microscopy DataBase and determine how the SP-TEM maps allow lipid visualization, the type of binding sites, the influence of symmetry, resolution and other factors. We ...
Peer Review File Description of Additional Supplementary Files Supplementary Movie 1 Reporting Summary Source data Source Data Rights and permissions Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and rep...
Software updates Release management Thermo Fisher Scientific offers a constant stream of software improvements and updates, ensuring you can achieve your goals as quickly and easily as possible. For Research Use Only. Not for use in diagnostic procedures....
Here, we review some of the complementary technical improvements that are helping sustain the cryo-EM revolution. We highlight advances in image processing that permit high resolution structure determination even in the presence of structural and conformational heterogeneity. We also review selected ...