与日益成熟的冷冻电镜单颗粒技术一同,冷冻电镜断层成像技术cryo-ET(cryo-electron tomography)和子断层图像平均法STA(sub-tomogram average)也正在悄然崛起。这两者将结构生物学引向解析柔性超大分子复合物结构、细胞原位蛋白结构等方向,应用范围广泛,覆盖蛋白复合物、病毒、细菌、细胞甚至组织,最高分辨率已达3Å ,是名...
Cryo-electron tomography (Cryo-ET) is a high-resolution technique that enables you to image a cell’s molecular machinery at physiological conditions. The in situ capabilities of cryo-electron tomography allow it to bridge the gap between single particle analysis (SPA) and structural cell ...
The Glacios 2 Cryo-TEM is ideally suited for single-particle analysis, cryo-electron tomography (cryo-ET), and micro-electron diffraction (MicroED) applications. Play Mute Current Time 0:00 / Duration -:- Loaded: 0% ShareFullscreen This is a modal window. This video is ...
The Krios G4 Cryo TEM is a highly stable 300 kV TEM platform suited for single particle cryo-EM, cryo electron tomography and microcrystal electron diffraction with atomic level resolution.
Recently, cryo TEM produces very exciting results of structural biology in combination with single particle analysis and electron tomography, since application field of the method expand to non crystalline samples or huge molecules. We developed a new generation cryo TEM, which achieves high throughput...
Applications Development Scientist Cryo-electron Tomography for Life Sciences 地点 Eindhoven, 荷兰 职位Id R-01279769 Your contributions will support directly the customer’s scientific endeavor as well as feed directly into new product development at Thermo Fisher. Experience interacting with new users ...
While the development of the cryo liftout solution was aimed at biological and battery technology, we have also seen it used for some unexpected applications. These include a TEM sample of toothpaste to look at the distribution of nano particles and a cryo tomography sample of ice-cream to inv...
展开 关键词: X-RAY-DIFFRACTION ELECTRON-MICROSCOPIC TOMOGRAPHY MINERALIZED TISSUES CONNECTIVE-TISSUE CORTICAL BONE IN-SITU DENTIN RESOLUTION SECTIONS PREDENTIN DOI: 10.1016/B978-0-12-416617-2.00009-6 被引量: 1 年份: 2013 收藏 引用 批量引用 报错 分享 全部...
Cryoelectron tomography provides unprecedented insights into the macromolecular and supramolecular organization of cells in a close-to-living state. Howeve... Rigort,Alexander,Bäuerlein,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 207发表: 201...
The Glacios 2 Cryo-TEM is ideally suited for single-particle analysis, cryo-electron tomography (cryo-ET), and micro-electron diffraction (MicroED) applications. Play Mute Current Time 0:00 / Duration 0:00 Loaded: 0% ShareFullscreen This is a modal window. The Playback API request ...