Cryo-EM sample preparation and data collection For each purified pilus sample, the concentration of the relevant T4P variant was normalized to 10 μg/ml, while the nanobodies were all at a final concentration of 50 μg/ml. 3 µl of the corresponding solution was applied to the car...
Cryo-EM sample preparation and data collection Quantifoil® type R2/1 holey carbon-coated support films on copper 200 mesh copper grids were used in order to analyze samples with cryo-EM. A PELCO easiGlow™ was used to glow discharge the grids at 0.4 mbar, grid negative, 15 mA and a...
Nanostructure Formation in the Lecithin/Isooctane/Water System, Naama Koifman, Maya Schnabel-Lubovsky, and Yeshayahu Talmon*, Department of Chemical Engineering and the Russell Berrie Nanotechnology Institute (RBNI), Technion-Israel Institute of Technology, Haifa 32000, Israel, the Journal of ...