Most biological specimens are too thick to be directly investigated and are therefore thinned by milling with a focused ion beam under cryogenic conditions (cryo-FIB). This procedure is prone to contaminations, which makes it a tedious process, often leading to suboptimal results. Here, we ...
Cryo Focused Ion Beam Milling Contact us Download flyer Overview Applications Solutions Resources Contact us Sample visualization and preparation with cryo-FIB milling FIB technology can help you prepare samples for a variety of imaging techniques, including cryo-electron tomography (cryo-ET), ...
Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy Nat Methods, 4 (2007), pp. 215-217 CrossrefView in ScopusGoogle Scholar 5 E. Villa, M. Schaffer, J.M. Plitzko, W. Baumeister Opening windows into the cell: focused-ion-beam milling for cryo-ele...
Cryo-electron tomography (cryo-ET) is a revolutionary technique for resolving the structure of subcellular organelles and macromolecular complexes in their cellular context. However, the application of the cryo-ET is hampered by the sample preparation step. Performing cryo-focused ion beam milling at...
Advances in high-pressure freezing and focused ion beam milling at cryogenic temperatures now allow for the routine preparation of thin (less than 200 nm) lamellae from cells or even small organisms8,9,10. Cryo-ET offers a unique opportunity to capture cellular processes in three dimensions ...
The iFLM Correlative System (Integrative Fluorescence Light Microscope) allows you to combine fluorescence imaging and ion milling within a single cryo-FIB SEM microscope. Explore applications Cryo-FIB for your lab Choose the best cryo-focused-ion-beam (cryo-FIB) for your lab...
参考文献 [1] S. Tackeet al., “A streamlined workflow for automated cryo focused ion beam milling,”bioRxiv, p. 2020.02.24.963033, Feb. 2020, doi: 10.1101/2020.02.24.963033.
Abstract: Cryo-focused ion beam milling of frozen-hydrated cells has recently provided unprecedented insights into the inner space of cells. In combination with cryo-electron tomography, this method allows access to native structures deep inside cells, enabling structural studies of macromolecules in sit...
Abstract: Cryo-focused ion beam milling of frozen-hydrated cells has recently provided unprecedented insights into the inner space of cells. In combination with cryo-electron tomography, this method allows access to native structures deep inside cells, enabling structural studies of macromolecules in sit...
Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography, Chyongere Hsieh1, Thomas Schmelzer2, Gregory Kishchenko1, Terence Wagenknecht1,3, and Michael Marko*,1,4; 1New York State Department of Health, Wadsworth Center, Empire State Plaza, Alb...