. The cryo-EM data were collected from the Electron Microscopy Laboratory and Cryo-EM Platform at Peking University. Initial cryo-EM screening was performed in part at the Center for Nanoscale Systems at Harvard University supported by the National Science Foundation under NSF award no. 1541959 ...
We thank the Core Facilities of Peking University School of Life Sciences for assistance with negative-staining electron microscopy; the Cryo-EM Platform of Peking University and the Electron Microscopy Laboratory of Peking University for cryo-EM data collection; and the High-performance Computing Platfo...
“Shuimu's advanced commercial cryo-EM service platform greatly supports the structural and functional study of biological macromolecules. I was lucky to collaborate with ShuimuBio to solve the three-dimensional structure of protein machines and unveil the mysteries of life activity.” ——Xiaodong Luan...
We thank the staff members at the cryo–EM centers of Westlake University and the School of Life Sciences of Peking University for their technical assistance with cryo–EM data collection, and the mass spectrometry platform of the National Facility for Protein Science in Shanghai Zhangjiang Lab. ...
2. Cryo-EM structures of the apo ClC-2 and its complex with highly specific inhibitor AK-42 were both determined at 3.5 Å resolution (Fig. 1a, Supplementary Figs. 3, 4a–c, Supplementary Table 1). These cryo-EM structures represented a homodimer of ClC-2 proteins. The overall ...
We thank the Tsinghua University Branch of China National Center for Protein Sciences (Beijing) for providing the cryo-EM facility support. We thank the computational facility support on the cluster of Bio-Computing Platform (Tsinghua University Branch of China National Center for Protein Sciences ...
Previous cryo-electron microscopy (cryo-EM) studies have determined the atomic structures of the USP14-free, substrate-engaged proteasomes at key functional steps, including ubiquitin recognition (states EA1and EA2), deubiquitylation (state EB), translocation initiation (states EC1and EC2) and proces...
Herein, we demonstrate the enhanced cryo-continuous rotation electron diffraction (cryo-cRED) method to address the beam damage issues of 3D COFs (Fig. 1). By combining hierarchical cluster analysis (HCA)43,44 with cryo-EM technique, the 3D ED data quality regarding the resolution and completene...
Fig. 1: Workflow of cryo-CLEM with one example using cryo-SIM. Vitrified cells on EM grids are too thick to be imaged directly by cryo-EM. Blind milling by cryo-FIB lacks the precision to target the region of interest (ROI), which needs to be solved by cryo-CLEM. The vitrified cells...
of interest is not guaranteed to be preserved when thinning the cell from several micrometers to less than 300 nm thick. Here, we report a cryogenic correlated light, ion and electron microscopy (cryo-CLIEM) technique that is capable of preparing cryo-lamellae under the guidance of three-...