Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions. Nat Biotechnol. 2017;35:463–74. Article CAS PubMed PubMed Central Google Scholar Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, et al. Genome-scale deletion screening of human long ...
GENESThe article focuses on a research by scientists Feng Zhang, Eric Lander and David Sabatini, which shows the use of the genome-editing system CRISPR for screening human cells for genes of interest and mentions the screening of genes involved in resistance to the chemotherapy drug etoposide....
Genome-wide CRISPR screen identifies MTA3 as an inducer of gemcitabine resistance in pancreatic ductal adenocarci 作者单位:天津医科大学肿瘤医院 IF:9.756 临床胰腺导管腺癌(PDAC)治疗失败的主要原因之一是产生吉西他滨(GEM)耐药。作者通过CRISPR/Cas9激活文库筛选发现MTA3介导了PDAC的GEM耐药,因此MTA3可能成为潜在...
Genome-wide CRISPR screen identifies MTA3 as an inducer of gemcitabine resistance in pancreatic ductal adenocarci 作者单位:天津医科大学肿瘤医院 IF:9.756 临床胰腺导管腺癌(PDAC)治疗失败的主要原因之一是产生吉西他滨(GEM)耐药。作者通...
Although both regions were uncovered in the AsCas12a screen with a single crRNA, screening with enAsCas12a strengthened this finding by three or four additional crRNAs, highlighting that the increased targeting space of enAsCas12a allows for more robust identification of drug resistance hotspots. ...
A separate team led by Eric Lander at the Broad Institute and David Sabatini at the Whitehead Institute for Biomedical Research in Cambridge, Massachusetts, used a library of 73,000 guide RNAs to screen for several genes, including those involved in resistance to the chemotherapy drug etoposide. ...
“Design of the gRNAs used within screens has a large impact on the quality of data output from a screen,” says Mollie Schubert, product manager, Integrated DNA Technologies (IDT). “If the selected guides are not efficiently editing the locus of interest, then you may suffer from false ne...
As mentioned earlier, having reliable results early in the drug discovery process is critical to reducing the financial risk associated with therapeutic development. The gRNA design in a library can have a profound effect on the outcome of a screen. ...
We tested this presumption by performing a genome-wide CRISPR screen aimed at identifying modulators of autophagy43,44 with a pool of all 19,820 T.spiezzo qgRNA plasmids, with the Brunello library14, and by comparing the results with a previous screen performed with the Cellecta library43. ...
We then set out to screen this gRNA library against a panel of 18 cell lines treated separately with each of 8 different drugs, or vehicle-treated control. The resulting relative abundance of gRNAs targeting each of thes...