Further studies to pinpoint are required to elucidate the underlying mechanism of the association between CRISPR and antibiotic resistance in these isolated strains.Md.Shahariar AlamSajib ChakrabortyTaibur RahmanMd.Ismail HosenAnik PaulA.K.M.Mahbub HasanM.Anwar Hossain医学微生物学(英文)...
Wastewater is among the main sources of antibiotic resistance genes (ARGs) in the environment, but effective methods to quickly assess ARGs on-site in wastewater are lacking. Here, using the typical ARG ermB as the target, we report a portable biosensor combining CRISPR/Cas12a and loop-mediated...
8. Jia, N., and Patel, D.J. (2022). Structure-based evolutionary relationship between IscB and Cas9.Cell research32, 875-877. 10.1038/s41422-022-00709-8. 9. Kamruzzaman, M., and Iredell, J.R. (2020). CRIS...
Widespread prescriptions of antibiotics and use in animal food production have led to a rising prevalence of antimicrobial resistance in the environment. Health experts predict that threats from antibiotic resistance could drastically increase in the coming decades, leading to some 10 million drug-resistan...
Antibiotic resistance has been unintentionally promoted through decades of antibiotic misuse and overuse. Under the driving pressure exerted by antibiotics, bacteria have evolved multiple strategies for resistance, while treatment alternatives have dwindled. This is because in contrast to the rapidly evolving...
gRNA Synthesis and Cloning Azenta will synthesize and clone your gRNA sequences as cDNA into your preferred vector regardless of vector size and complexity, using the same industry-leading proprietary technology that Azenta has developed for standard gene synthesis products. ...
Transduced cells were selected using puromycin (2 μg ml−1 for K562 cells) starting 48 h post-transduction and over the course of 7 days with daily addition of the antibiotic. After 24 h growth in puromycin-free medium, 0.1 million cells were collected and total RNA was ...
With this system, EcN needs to maintain the plasmid to provide InfA and survive. The antibiotic resistance gene was not removed from the plasmid to allow for CFU quantification in future experiments using mixed strains. Expressing InfA on the gRNA plasmid eliminated the dependence on ABX but ...
Chromosomal mutations involved in antibiotic resistance in Staphylococcus aureus Front Biosci, 4 (2012), pp. 900-915, 10.2741/s307 View in ScopusGoogle Scholar [82] L. Cullen, S. McClean Bacterial adaptation during chronic respiratory infections Pathogens, 4 (2015), pp. 66-89, 10.3390/pathogen...
Abstract: Objective We aimed to characterize the CRISPR-Cas systems in clinical hypervirulent K. pneumoniae isolates and to investigate the potential association of CRISPR-Cas systems with virulence and antibiotic resistance. Methods A total of 61 hypervirulent K. pneumoniae strains were collected...