We also discuss the existing problems and perspectives of CRISPR/Cas9-based genomic editing in plants. 展开 关键词: plants CRISPR/Cas9 targeted mutations genotyping DOI: 10.16288/j.yczz.15-395 被引量: 1 年份: 2016 收藏 引用 批量引用 报错 分享 ...
Technical and ethical problems associated with the use of CRISPR/Cas for editing human embryonic genomes are also discussed.doi:10.1134/S1062360422040075A. A. Shmakovaab*O. P. ShmakovacA. A. KarpukhinaaY. S. Vassetzkya**Pleiades PublishingRussian Journal of Developmental Biology...
However, while it is considered as one of the most precise gene-editing tools, CRISPR suffers problems of off-target effects that can affect non-targeted DNA sequences elsewhere in the genome. This is a big problem for the modification of human cells, and can result in the creation of doub...
植物CRISPRCas9基因组编辑系统与突变分析
Nevertheless, problems in editing plant genomes continue to be thoroughly understood and approach investigated. In conclusion, CRISPR/Cas-based technologies have emerged as powerful tools in reshaping modern agriculture in terms of breeding for resistance, elucidating host鈥損athogen interactions, production...
Over the past decade, CRISPR has become as much a verb as it is an acronym, transforming biomedical research and providing entirely new approaches for dissecting all facets of cell biology. In cancer research, CRISPR and related tools have offered a window into previously intractable problems in ...
[170,171,172]. CD45-targeting CAR T-cells used for hematologic tumors faces more problems. CD45 is generally expressed in blood cells including T cells and hematopoietic stem cells, so CD45 CAR T-cell may cause fratricide and severe hematopoietic toxicity. On the other hand, CD45 influences...
Over the past decade, CRISPR has become as much a verb as it is an acronym, transforming biomedical research and providing entirely new approaches for dissecting all facets of cell biology. In cancer research, CRISPR and related tools have offered a window into previously intractable problems in ...
Due to the speed of research, a solution to these problems and complications will be also provided. Challenges of CRISPR/Cas9 Delivery challenge Even though CRISPR/Cas9 may be a developed gene editing innovation that has been utilized broadly, it keeps up numerous issues due to the off-target ...
The traditional gene editing technology randomly integrates a target gene into a receptor genome, thus producing results with poor predictability and problems, such as gene silencing and unexpected variations. The targeted gene editing technology can precisely modify the locus information of a genome, ac...