I'm excited to tell you about a new kind of collaboration that will absolutely create solutions to these big problems. It's a collaboration that's unexpected because it's between humans and the tiniest organisms that populate our planet: the bacteria ...
significant challenges,such as editing efficiency,relative difficulty in delivery,off-target effects,immunogenicity,etc.This article summarizes the applications of CRISPR-Cas from bench to bedside and highlights the current obstacles that may limit the usage of CRISPR-Cas systems as gene-editing toolkits...
Herein, we will review the development and applications of the exciting uses of the CRISPR/Cas9 technique for cancer research and therapy with focus on origin, progress, clinical trials, implications, and challenges ahead. Major ethical and safety concerns are perhaps unknown long term consequences ...
application of this technique is still relatively rare in stem cell research. There are numerous specialized challenges in applying large-scale useful genomics approaches to differentiated stem cell populations. Here, we present the first comprehensive review on CRISPR-based functional genomics screening in...
The DBTL cycle has four components: (i) ‘design’ identifying problems, selecting host and pathway, (ii) ‘build’ assembling building blocks into the host, (iii) ‘test’ validating the strain for target metabolite production and (iv) ‘learn’ data analysis and informing iterative cycles. ...
Here, we review the recent advances in CRISPR/Cas9 technology and the ways that have been adopted to expand our capacity for precise genome manipulation... Y Mei,Y Wang,H Chen,... - 《遗传学报》 被引量: 0发表: 2016年 Facing the Challenges of CRISPR-Cas9 Technology in Genome Editing CRI...
Over the past decade, CRISPR has become as much a verb as it is an acronym, transforming biomedical research and providing entirely new approaches for dissecting all facets of cell biology. In cancer research, CRISPR and related tools have offered a window into previously intractable problems in ...
onset are expected to serve as identifying factors for targeted delivery or targets for gene editing. Diseases are both varied and complex, and the choice of appropriate gene-editing methods and delivery vectors for different diseases is important. Meanwhile, there are still many potential challenges...
Paul, J.W., Qi, Y. CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects.Plant Cell Rep35, 1417–1427 (2016). https://doi.org/10.1007/s00299-016-1985-z Download citation Received30 January 2016 Accepted Issue DateJuly 2016 ...
(HSCs)ex vivoand to treat a wide variety of diseases and disordersin vivo. Nevertheless, the clinical translation of this unique technology still faces many challenges, especially targeting, safety and delivery issues, which require further improvement and optimization. In addition, with the outbreak...