E. Brown, 通过外植体离体培养获得无菌组培苗用于遗 传转化.分别取湖北地黄的根,茎,嫩叶,老叶,蕾 和花6个不同部位的新鲜样品于液氮中速冻.设3次生 物学重复.置于–80°C冰箱保存, 备用. CRISPR/Cas9基因编辑载体pKSE401 (Xing et al., 2014)由中国农业大学陈其军教授惠赠.大肠杆菌 (Escherichia coli)感受态...
Table S3. Selected genes for pilot study to deduce optimal targetable region Table S4. List of sgRNAs and their target in the B. cenocepacia K56-2 essential genome Table S5. Relative abundance of the CRISPRi knockdown mutants in the pooled librariesShow all extrasVolume...
The muscle was carefully dissected out and snap-frozen in liquid nitrogen-cooled isopentane in the optimal cutting temperature (OCT) compound (Sakura Finetek Inc., Torrance, CA, USA) for morphological analysis. Morphological analysis General muscle histopathology was revealed with hematoxylin and eosin...
CRISPETa - [python, webserver] - A flexible tool to design optimal pairs of sgRNAs for deletion of desired genomic regions. CRISpick - [webserver] - CRISPick, developed by the Broad Institute, ranks and picks candidate CRISPRko/a/i sgRNA sequences to maximize on-target activity for target...
CRISPRLearner, the system presented in this paper, makes it possible to predict the on-target cleavage efficiency (also called on-target knockout efficiency) of a given sgRNA sequence, specifying the target genome that this sequence is designed for. After efficiency prediction, the researcher can ...