张锋实验室的Target Finder:http://crispr.mit.edu/ Michael Boutros实验室的E-CRISP:http://www.e-crisp.org/E-CRISP/designcrispr.html CasFinder:http://arep.med.harvard.edu/CasFinder CRISPR Optimal Target Finder:http://tools.flycrispr.molbio.wisc.edu/targetFinder/ Broad研究院的CRISPR专家John Doenc...
You can use the flyCRISPR Optimal Target Finder tool to identify highly specific target sites in the Drosophila genome. Due to the frequency of naturally occurring SNPs between fly strains, it is a good idea to sequence the region you intend to target in the fly strain you plan to inject i...
张锋实验室的Target Finder:http://crispr.mit.edu/ Michael Boutros实验室的E-CRISP:http://www.e-crisp.org/E-CRISP/designcrispr.html CasFinder:http://arep.med.harvard.edu/CasFinder CRISPR Optimal Target Finder:http://tools.flycrispr.molbio.wisc.edu/targetFinder/ 第三步,将gRNA克隆到质粒中,导入...
You can use the flyCRISPR Optimal Target Finder tool to identify highly specific target sites in the Drosophila genome. Due to the frequency of naturally occurring SNPs between fly strains, it is a good idea to sequence the region you intend to target in the fly strain you plan to inject i...
Considering polymorphisms between a chosen fly strain and the reference genome,flyCRISPR Optimal Target Finderidentifies gRNA target sites in user-supplied DNA sequence, evaluated with the reference of genome sequences, includingD. melanogasterrelease 6,D. melanogastervas-Cas9(III) (BDSC#51324) whole-...
Zhu H, Misel L, Graham M, Robinson ML, Liang C (2016) CT-finder: a web service for CRISPR optimal target prediction and visualization. Sci Rep 6:1–8. https://doi.org/10.1038/srep25516 Article CAS Google Scholar Zhu Y, Klompe SE, Vlot M, van der Oost J, Staals RHJ (2018) Sh...
CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization Article Open access 23 May 2016 The GeneCards Suite Chapter © 2021 Keywords CRISPR/Cas9 Guide RNA design Inducible guide RNAs Transcriptional activity DNA modification Genomic loci Search...
a significant consideration is that the extent of stem cell differentiation induced can significantly impact the final analysis. Hence, establishing optimal maturation conditions is essential to ensure that all stem cells are effectively guided to differentiate into the desired target cells [145]. Additio...
Zhang D, Hurst T, Duan D, Chen SJ (2019) Unified energetics analysis unravels SpCas9 cleavage activity for optimal gRNA design. Proc Natl Acad Sci 116(18):8693–8698 CAS PubMed PubMed Central Google Scholar Zhu LJ, Holmes BR, Aronin N, Brodsky MH (2014) CRISPRseek: a bioconductor pa...
A major challenge for effective application of CRISPR systems is to accurately predict the single guide RNA (sgRNA) on-target knockout efficacy and off-target profile, which would facilitate the optimized design of sgRNAs with high sensitivity and specif