在本研究中，作者利用CRISPR-cas9在内的多种生物信息学工具识别了与Hippo信号通路相关的基因及其对癌症风险的潜在影响。具体研究如下：本文的亮点是使用了Depmap数据库的CRISPR-cas9 screening data，得到的基因是与细胞增殖显著相关的基因，与直接用Hippo通路效果更好。其他的分析则都很普通。结合Depmap数据库进行生信分析...
本文的亮点是使用了Depmap数据库的CRISPR-cas9 screening data,得到的基因是与细胞增殖显著相关的基因,与直接用Hippo通路效果更好。其他的分析则都很普通。 结合Depmap数据库进行生信分析,欢迎咨询小编! 研究结果: 确定低级别胶质瘤中功能基因组 作者首先寻找LGG差异表达基因(图A)。接着,作者还探究了癌基因根据其对其...
LentiPool CRISPR Libraries empower CRISPR-Cas9 screening without the need for high-throughput infrastructure. These high-quality pooled lentiviral libraries cover the same gene targets as our LentiArray libraries and are delivered as ready-to-use high-titer (>1 x 108TU/mL) lentivirus...
LentiPool CRISPR Libraries empower CRISPR-Cas9 screening without the need for high-throughput infrastructure. These high-quality pooled lentiviral libraries cover the same gene targets as our LentiArray libraries and are delivered as ready-to-use high-titer (>1 x 108 TU/m...
CRIPSR/Cas9 Screening by GeCKO-V2 1. 评估GeCKO-V2质粒文库的好坏 样本比对信息统计表 gRNA的read count 分布图 均一性斜率图 2 利用MageCK分析CRISPR文库筛选数据 下载CRIPSR-Screening library list 合并双端测序fastq文件 计算样本中gRNA的均一化counts 使用Mageck mle计算不同样本间组合情况 ...
全基因组CRISPR/Cas9高通量筛选人体细胞中的功能基因组学 英文名:《Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells》 简单说就是利用CRISPR/Cas9技术对人类的基因组(23对染色体上的全部基因,大约两万个)...
A subset of CRISPR/Cas9 knockout screening data on melanoma cell lines
Genome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies across cancer cell lines. Current CRISPR-Cas9 knockout libraries, however, primarily target protein-coding genes. T
To facilitate the iterative knockout of more than three secreted proteins, we performed a pooled CRISPR-Cas9 knockout library to measure the essentiality of all secreted proteins. This knowledge of gene essentiality and new capability for pooled screening methods should also inform future efforts to...
Genome-wide functional screening using the CRISPR-Cas9 system is a powerful tool to uncover tumor-specific and common genetic dependencies across cancer cell lines. Current CRISPR-Cas9 knockout libraries, however, primarily target protein-coding genes. T