CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9)-based gene editing is a robust tool for functional genomics research and breeding programs in various crops. In soybean, a number of laboratories have obtained mutants by CRISPR/Cas9 system; however, there ...
CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engine...
脊尾白对虾(Exopalaemon carinicauda)是一种重要的经济类甲壳动物,且已经实现了CRISPR/Cas9基因编辑体系。在本研究中,研究人员首先分离鉴定到一条脊尾白虾胰岛素样肽基因EcILP,经分析其属于DILP7/relaxin型ILP基因,进一步利用CRISPR/Cas9基因...
Dear Editor,CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated)-based genome editing is a powerful and widely adopted technology for introducing specific mutations in animal and plant systems. Typically, a CRISPR/Cas9 system is composed of the endonuclease protein ...
Cyagen’s CRISPR Cas9 gene editing technology services can generate custom mice and rats – including knockout, knockin, and humanization models. 3,000+ university and company partners.
CRISPR/Cas9在农作物改良中的应用 引言 基因组编辑技术是近些年新发展起来的一种对基因组进行精准修饰的技术,它能在基因组上进行定点突变、定点插入或删除、基因置换、在两位点或多位点同时定点突变或小片段缺失等操作,已经成为目前发育生物学中的重要工具。该技术可以用于系统地研究基因、调控元件在特定生理或发育过程中...
et al. CRISPR–Cas9 gene editing for sickle cell disease and β-thalassemia. N. Engl. J. Med. 384, 252–260 (2021). Wang, L. et al. Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies. Cell Res. 30, 276–278 (2020). Article Google ...
近日,Plant Biotechnology Journal在线发表了华南农业大学国家大豆改良中心广东分中心研究团队题为“CRISPR/Cas9-mediated gene editing of GmJAGGED1 increased yield in the low latitude soybean variety Huachun 6”的研究论文,该研究利用CRISPR/Cas9技术对高产高蛋白大豆品种华春6号的GmJAGGED1及其同源基因进行定点编辑创...
CRISPR-Cas9 belongs to type II in the second class of single-protein effector modules and is currently the most widely used and thoroughly studied genome editing tool. Multiple type II systems have been developed as efficient gene editing tools for DNA or RNA and applied to animals, plants, an...
[19]Li C,Nguyen V,Liu J,et al. Mutagenesis of seed storage protein genes in soybean using CRISPR/Cas9[J]. BMC Research Notes,2019,12(1):176. [20]Sun L,Williams J S,Li S,et al. S-locus F-box proteins are solely responsible for S-RNase-based self-incompatibility of Petunia pollen...