2025年5月26日,《Plant Biotechnology Journal》发表了一项令人振奋的研究成果:‘’A highly efficientCRISPR-Cas9-based gene-editing system in oat (Avena sativa L.)‘’,加拿大麦吉尔大学Jaswinder Singh团队在燕麦中首次成功实现了CRISPR-Cas9基因编辑,
在进行基因组编辑时,CRISPR-Cas9系统起作用的主要是2个部分:是嵌合的引导RNA(gRNA,chimeric guide RNA),在识别靶序列的过程中起决定性作用:另一个是具有核酸酶活性的CRISPR-Cas9蛋白,Cas9上的几个特异碱基,即前间隔相邻基序(protospacer adjacent motif,PAM)也参与识别靶序列。PAM序列是有物种特异性的,不同细菌中序...
近日,华中农业大学洪登峰教授团队在aBIOTECH发表了题为"Mutation of ERECTA homologous genes confers ideal plant architecture in Brassica napus"的研究论文。该研究通过CRISPR/Cas9技术对甘蓝型油菜BnaER、BnaERL1和BnaERL2基因进行编辑,成功创制...
当Vertex/CRISPR Therapeutics的Casgevy(exagamglogene autotemcel)在2023年成为首个获FDA批准的CRISPR-Cas9基因编辑疗法时,整个生物医药界为之振奋。这款针对镰状细胞病(SCD)和β-地中海贫血的突破性药物展现出近乎治愈的疗效,但背后却是患者需要经历造血干细胞采集、体外编辑、化疗预处理等一系列复杂流程,单次治疗成...
CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engine...
Overall, these results showed that mutations were, for the first time, induced by CRISPR-based gene editing approach in peanut. This research demonstrated the potential application of gene editing for mutagenesis in peanut and suggested that CRISPR/Cas9 technology may be useful in the peanut breeding...
CRISPR/Cas9-gene editing approaches in plant breedingHimanshu SainiRajneesh ThakurRubina GillKalpana TyagiManika Goswami
(编辑推荐)这篇综述系统阐述了纳米颗粒作为CRISPR/Cas9递送载体的突破性优势:可搭载大尺寸Cas9酶和复杂向导RNA(gRNA),实现传统方法难以编辑的基因组区域;其可规模化生产的特性(如聚合物/脂质纳米粒)兼具组织靶向性和光热调控潜力(如金纳米颗粒),为遗传病和癌症治疗提供了高效低毒的递送方案。
Tackling in vivo screening complexity Lei Tang Research Highlights11 Feb 2025 Nature Methods Volume: 22, P: 224 Expanded toolkits for RNA circularization Optimized methods for the synthesis of circular RNA in vitro and in cells, and a complementary Cas9 de-immunization method, enhance RNA per...
Cas9实验步骤教程 1. Copy the target sequence (<500bp), paste in “query sequence:” of the webpage: https://crispr.cos.uni-heidelberg.de/ 2. Set two parameters: ① target site length: 20 ② species: Arabidopsis thaliana TAIR10 3. Click “Submit”, pick the best 20-bp “target_seq...