CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9)-based gene editing is a robust tool for functional genomics research and breeding programs in various crops. In soybean, a number of laboratories have obtained mutants by CRISPR/Cas9 system; however, there ...
图1CRISPR/Cas9在支气管上皮细胞中的研究进展Figure 1CRISPR/Cas9 in bronchial epithelial cells. A: targeting theMUC18gene with a viral vector down-regulates the inflammatory response in epithelial cells; knockdown of theS...
CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engine...
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Prepare at least 0.5x more of the Cas9/gRNA complexes for electroporation than needed to avoid introducing bubbles. Design When doing your first editing experiment, we recommend performing this positive con...
CRISPR/Cas9在农作物改良中的应用 引言 基因组编辑技术是近些年新发展起来的一种对基因组进行精准修饰的技术,它能在基因组上进行定点突变、定点插入或删除、基因置换、在两位点或多位点同时定点突变或小片段缺失等操作,已经成为目前发育生物学中的重要工具。该技术可以用于系统地研究基因、调控元件在特定生理或发育过程中...
Gene expression is regulated by multiple processes, and the translation of mRNAs into proteins is an especially critical step. Upstream open reading frames (uORFs) are widespread cis-elements in eukaryotic genes that usually suppress the translation of d
近日,Plant Biotechnology Journal在线发表了华南农业大学国家大豆改良中心广东分中心研究团队题为“CRISPR/Cas9-mediated gene editing of GmJAGGED1 increased yield in the low latitude soybean variety Huachun 6”的研究论文,该研究利用CRISPR/Cas9技术对高产高蛋白大豆品种华春6号的GmJAGGED1及其同源基因进行定点编辑创...
et al. CRISPR–Cas9 gene editing for sickle cell disease and β-thalassemia. N. Engl. J. Med. 384, 252–260 (2021). Wang, L. et al. Reactivation of γ-globin expression through Cas9 or base editor to treat β-hemoglobinopathies. Cell Res. 30, 276–278 (2020). Article Google ...
Genome editing is a valuable technique for gene function analysis and crop improvement. Over the past two years, the CRISPR-Cas9 system has emerged as a powerful tool for precisely targeted gene editing. In this study, we predicted 11 U6 genes in soybean