Workflow of mouse genetic engineering with CRISPR/Cas9 technology Select target site and design sgRNA, design homologous recombination vector (optional) Prepare sgRNA and Cas9 mRNA, generate homologous recombination vector (optional) Microinject sgRNA and Cas9 mRNA (and homologous recombination vector (opti...
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 is a genome-editing technology1,2 that utilizes archaeal and bacterial Cas9 nucleases to introduce double-stranded breaks in DNA at targeted sites. These breaks can be used to remove, replace, or add pieces of DNA. While not...
使用CRISPR-Cas9 切割 DNA 后,双链断裂可以通过两种细胞自然修复机制之一进行修复(图 3)。 在不存在修复模板的情况下,非同源末端连接(NHEJ)过程会导致 gRNA 定义的断裂周围有不同插入或缺失(插入缺失)的异质细胞群。可利用该过程在特定序列周围生成有随机缺失的细胞系,从而获得功能性敲除。
molecular level. However, the application of CRISPR/Cas9 technology is not without controversy. The rapid development of technology brings with it multiple challenges such as ethics, law, and social acceptance. Issues such as the long-term e...
引用本文:李仪扬,周执政,王淑菲等.CRISPR/Cas9基因编辑技术在疾病治疗中的应用与展望[J].生物技术进展,2025,15(01):35-42. (LI Yiyang,ZHOU Zhizheng,WANG Shufei,et al.Application and Prospect of CRISPR/Cas9 Gene Editing Technology in Diseas...
CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the double strand break can result in insertion or deletion mutations, while repair pathways can be engine...
JA. Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing. Mol Cancer. 2022;21(1):78.Huang S, Yan Y, Su F, et al. Research progress in gene editing technology. Front Biosci (Landmark Ed). 2021;26(10):916-927.Li T, Yang Y, Qi H, et al. CRISPR/Cas9 ...
基因编辑(Gene editing),是一种新兴的比较精确的能对生物体基因组特定目标基因进行修饰的一种基因工程技术。基因编辑能够高效率地进行定点基因组编辑,故而在基因研究、基因治疗和基因育种等方面展示出了巨大的应用前景。 迄今为止,CRISPR/Cas9作为一种高效的基因编辑工具,广泛应用于科学界。基因编辑是一种对基因组序列...
华中农业大学农业微生物国家重点实验室李英俊团队近期在Engineering Microbiology杂志上发表了题为“CRISPR-based gene editing technology and its application in microbial engineering”的综述论文,系统梳理了不同CRISPR-Cas系统的原理以及其在微...
EDITGENE provides various cell lines gene editing services and related products, such as CRISPR library screening, knockout cell line, Knock in cell line, point mutation cell line Cas12a, etc.