Recent genome editing approach of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system is a leading, powerful, versatile, ground-breaking, and smart plant genome editing tool with greater efficiency for designing of stress resilient crops. CRISPR/Cas ...
Niu Q, Wu S, Li Y, Yang X, Liu P, Xu Y, Lang Z (2020) Expanding the scope of CRISPR/Cas9-mediated genome editing in plants using an xCas9 and Cas9-NG hybrid. J Integr Plant Biol 62:398–402 Schardl CL, Byrd AD, Benzion G, Altschuler MA, Hildebrand DA et al (1987) Design...
此外，该研究还发表了多篇论文，包括2019年发表的《Single transcript unit CRISPR 0 systems for robust Cas9 and Cas12a mediated plant genome editing》和2016年发表的《A single transcript CRISPR-Cas9 system for efficient genome editing in plants》，进一步证明了STU-SR系统在植物基因组编辑领域的卓越表现。
近日,福建农林大学林学中心顾连峰教授课题组在New Phytologist发表了题为Bamboo mosaic virus-mediated transgene-free genome editing in bamboo的研究论文(https://doi.org/10.1111/nph.20386)。该研究首先构建了能够同时递送SpCas9和sgRNA的病毒载体pBaMV-Cas9,初步建立了一种基于BaMV的CRISPR/Cas非转基因的竹子基因编...
The CRISPR/Cas system has been harnessed to achieve efficient genome editing in a variety of organisms, including bacteria, yeast, plants, and animals, as well as human cell lines [12]-[27]. More importantly, using this RNA-guided endonuclease technology, multiple gene mutations and their ...
The CRISPR-Cas system for plant genome editing: Advances and opportunities Here, we provide a detailed overview of the CRISPR-Cas system and its adoption in different organisms, especially plants, for various applications. ... V Kumar,M Jain - 《Journal of Experimental Botany》 被引量: 115发表...
The development of new CRISPR–Cas genome editing tools continues to drive major advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents—nucleases, base editors, transposases/recombinases and prime editors—are currently available for modifying genomes in experimental sy...
此外, An等人[36]以银腺杨(Populus alba×glandulosa '84K')为 材料, 使用高活性的MAS启动子驱动Cas9系统, 使突变 表 1 CRISPR/Cas基因组编辑技术在木本植物性状改良中的应用简表(详见表S1) Table 1 Summary of the application of CRISPR/Cas genome editing in woody plant traits improvement (see Table ...
CRISPR/Cas9-mediated mutagenesis of phytoene desaturase in diploid and octoploid strawberry. Plant Methods 15, 45. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ (2014). A CRISPR/Cas9 toolkit for multiplex genome editing in plants. BMC Plant Biol 14, 327. Xu...
et al. DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins. Nat. Biotechnol. 33, 1162–1164 (2015). Article CAS PubMed Google Scholar Osakabe, Y. et al. CRISPR-Cas9-mediated genome editing in apple and grapevine. Nat. Protoc. 13, 2844–2863 (2018). ...