plant genome editingprime editingprime editing-derived techniquesThe clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system is a fast-growing,genome editing technology that has wide applications in identifying gene functions as well as improving agricultural ...
Plant Biotechnology Reports (2024) Genome editing based trait improvement in crops: current perspective, challenges and opportunities Surender Singh Roni Chaudhary Siddharth Tiwari The Nucleus (2024) Current approaches and future potential for delivering CRISPR/Cas components in oilseeds and millets Sa...
Genome editing by the CRISPR/Cas9 system is usually implemented via the generation of transgenic plants carrying Cas9 and sgRNA genes in the genome. Transgenic plants are usually developed by in聽vitro regeneration from single transformed cells, which requires using different in聽vitro culture-based ...
最后,前几天超市打折,华南农的校友们,冲了吗?! 参考文献:Xie X, Ma X, Zhu Q, Zeng D, Li G, Liu YG. CRISPR-GE: A Convenient Software Toolkit for CRISPR-Based Genome Editing. Mol Plant. 2017 Sep 12;10(9):1246-1249. doi: 10.1016/j.molp.2017.06.004. Epub 2017 Jun 15. PMID: 28624...
2024年5月7日,Plant Biotechnology Journal(IF:13.8)在线发表了西南大学张勇、马里兰大学戚益平、扬州大学张韬团队合作的最新成果:“Expanding plant genome editing scope and profiles with CRISPR-FrCas9 systems targeting palindromic TA sites”,本文深入探讨了CRISPR-FrCas9系统在植物基因组编辑中的应用与优化,FrCas9...
Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells. Nat. Methods 12, 237–243 (2015). CAS PubMed Google Scholar Zuris, J. A. et al. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat....
A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing CRISPR/Cas has emerged as potent genome editing technology and has successfully been applied in many organisms, including several plant species. However, delivery of genome editing reagents remains a challenge in...
Plant biotechnology is now entering a new phase where random mutagenesis methods, such as EMS mutagenesis and γ-radiation, are being superseded by genome editing technologies that enable precise manipulation of specific genomic sequences. Such technologies rely on sequence-specific nucleases (SSNs), ...
Zhou, J., Deng, K., Cheng, Y., Zhong, Z., Tian, L., Tang, X., Tang, A., Zheng, X., Zhang, T., Qi, Y. and Zhang, Y. (2017) CRISPR-Cas9 based genome editing reveals new insights into MicroRNA function and regulation in rice. Front Plant Sci8, 1598. ...
The transgene free potato plants showed mutations in coilin gene that conferred resistance to RNA based potyvirus Y (PVY) (Makhotenko et al., 2019). CRISPR/Cas mediated genome editing laid a way for understanding the plant virus interactions and development of immunity against single or multiple...