The ease and efficacy of CRISPR/Cas9 germline gene editing in animal models paved the way to human germline gene editing (HGGE), by which permanent changes can be introduced into the embryo. Distinct genes can be knocked out to examine their function during embryonic development. Alternatively, ...
Hence results from NGS diagnosis could be used as a base by CRISPR/Cas tool for editing the mutated gene. CRISPR/Cas9 genetic editing technology has been successfully employed for gene knock-in, gene knock-out, gene repair, and transcriptional regulation [10]. In this review, we have ...
Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes...
RNA-Guided Genome Editing for Target Gene Mutations in Wheat The clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas) system has been used as an efficient tool for ... SK Upadhyay,J Kumar,A Alok,... - 《G3 Genes Genomes Genetics》 被引量...
have drawn attention in engineering and development for use in gene editing [31,32]. Consequently, most of the CRISPR/Cas proteins are derived fromStaphylococcus aureusandStreptococcus pyogenes[33]. A prominent version of Cas9 that has been used has evolved inStreptococcus pyogenes, therefore referred...
CRISPR-mediated base editing can introduce single-nucleotide changes in the DNA of living cells. One intriguing application of base editing is to screen pivotal amino acids for protein function in vivo; however, it has not been achieved. Here, we report
(Gene); DH5α感受肽细胞、血液/组织/细胞基因组DNA提取试剂盒、琼脂糖凝胶回收试剂盒(天根生物); BbsⅠ核酸内切酶、EnGen™突变检测试剂盒、T4快速连接试剂盒、Q5 Hotstar高保真多聚核酸酶、T7E1核酸内切酶(NEB); Premix Taq(TaKaRa); 蛋白Marker (Thermo); ECL发光液(Millipore); 兔源G6PD抗体(CST); 兔源...
Functional correction of large factor VIII gene chromosomal inversions in hemophilia A patient-derived iPSCs using CRISPR-Cas9. Cell Stem Cell, 2015, 17: 213–220 33 DeWitt M A, Magis W, Bray N L, et al. Selection-free genome editing of the sickle mutation in human adult hematopoietic ...
The evolution of CRISPR tools that have been harnessed in the investigation of cancer biology. Since the inception of CRISPR-associated 9 (Cas9) gene editing in mammalian cells, there has been a rapid expansion in the field of CRISPR technology. This expansion has led to the development of var...
Complete population collapse of malaria vector Anopheles gambiae in cages is achieved using a gene drive that targets doublesex. In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-