The ease and efficacy of CRISPR/Cas9 germline gene editing in animal models paved the way to human germline gene editing (HGGE), by which permanent changes can be introduced into the embryo. Distinct genes can be knocked out to examine their function during embryonic development. Alternatively, ...
Hence results from NGS diagnosis could be used as a base by CRISPR/Cas tool for editing the mutated gene. CRISPR/Cas9 genetic editing technology has been successfully employed for gene knock-in, gene knock-out, gene repair, and transcriptional regulation [10]. In this review, we have ...
class 2 Cas systems, of which the Cas9 nuclease is the first and most extensively used, have drawn attention in engineering and development for use in gene editing [31,32].
Applications of the CRISPR-Cas9 system to edit the genome have widely expanded to include DNA gene knock-out, deletions, chromosomal rearrangements, RNA editing and genome-wide screenings. Here we show the application of CRISPR-Cas9 technology to edit the mouse and human immunoglobulin (Ig) genes...
Complete population collapse of malaria vector Anopheles gambiae in cages is achieved using a gene drive that targets doublesex. In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-
Functional correction of large factor VIII gene chromosomal inversions in hemophilia A patient-derived iPSCs using CRISPR-Cas9. Cell Stem Cell, 2015, 17: 213–220 33 DeWitt M A, Magis W, Bray N L, et al. Selection-free genome editing of the sickle mutation in human adult hematopoietic ...
(Gene); DH5α感受肽细胞、血液/组织/细胞基因组DNA提取试剂盒、琼脂糖凝胶回收试剂盒(天根生物); BbsⅠ核酸内切酶、EnGen™突变检测试剂盒、T4快速连接试剂盒、Q5 Hotstar高保真多聚核酸酶、T7E1核酸内切酶(NEB); Premix Taq(TaKaRa); 蛋白Marker (Thermo); ECL发光液(Millipore); 兔源G6PD抗体(CST); 兔源...
interspaced short palindromic repeats (CRISPR) in association with Cas nuclease stands out from other gene-editing methods, such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), due to simplicity, high fidelity, and multi-target editing potential [6]. ...
Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)gene-editing technology is the ideal tool of the future ... T Li,Y Yang,H Qi,... - 信号转导与靶向治疗(英文) 被引量: 0发表: 2023年 CRISPR/Cas genome editing in grapevine:recent advances,challe...
Esvelt, K.M. et al. Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.Nat. Methods10, 1116–1121 (2013). ArticleCASPubMedPubMed CentralGoogle Scholar Hou, Z. et al. Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.Proc. Natl...