即先把目的基因(gene of interest)沉默掉(在前方加一个终止子),通过把stop拿掉而条件性激活。 (3) Cre构建报告小鼠 reporter mouse。比如,想让Gli1+ cells 都绿的发慌。首先介绍小鼠基因组内两个外源性基因的安全敲除位点:Rosa26和H11。Rosa26是小鼠6号染色体上由3个外显子构成的非编码基因,易插入外源基因,易...
system,utilization of hybridheterosis,transgenic male sterility tapetal-specificpromoter,barnase IV !!主堂垡笙苎 生型竺!塞竺茎堡墨竺垄堕塑苎至蔓塑墨壁垡茎生堕型星旦壅查苎奎些!!兰一 1.前言 1.I研究的目的和意义 杂种优势是生物界的一种普遍现象,利用杂种优势大幅度提高产量是20世纪作物 ...
LoxP System in Transgenic Mice ZHU Huan2Zhang SHI Jing2 Quan Department ofPathology Third Military Medical University Chongqing400038 China Abstract Cre site2specific DNA recombinase system has been developed as a novel powerful tools for manipulating DNA bothin vitroandin vivo It has been used in ...
上海200092) 关键词Cre重组酶;Loxp位点;转基因技术;Cre/Loxp系统中图分类号Q812文献标识码:A文章编号:1008⁃1836(2008)02⁃0078⁃03 随着分子生物学的迅猛发展,转基因技术发挥着越来越重要的作用,已经渗透到人类生活的各个领域。
Cre/loxP system has long been recognized as the best SSR system for genome engineering, as it is effective not only in bac- teria but also in eukaryotic cells. Depending on the location and orientation of the loxP sites, the Cre-mediated recombination between the loxP sites can lead to a ...