1、A Model Study:Simulation of Marker Gene Deletion and Target Gene Replacement Mediated by Cre/lox System in Cell Line *CUI Wen-Tao 1,2,REN Li-Ming 1,HOU Jian 1,ZHANG Ying 1,CHEN Yong-Fu 1,AN Xiao-Rong 1*(1State Key Laboratory for Agrobiotechnology,China Agricultural University,...
免费在线预览全文 一种利用Cre_loxP系统进行标记基因删除与靶基因置换的细胞模型研究_英文_.pdf ResearchPapers 研究报告 生物化学与生物物理进展 ProgressinBiochemistryandBiophysics 2008,35(6):650~660 AModelStudy:SimulationofMarkerGene DeletionandTargetGeneReplacement * MediatedbyCre/loxSysteminCellLine 1,2) ...
Saito, K. Kobayashi, Gene therapy for hepatocellular carci- noma using two recombinant adenovirus vectors with alpha- fetoprotein promoter and Cre/lox P system, J. Virol. Methods 92 (2001) 5-17.Sakai Y, Kaneko S, Sato Y, et al. Gene therapy for hepatocellular carcinoma using two ...
This system can be implemented without inserting any extra supporting proteins or sequences. The Cre and the original Lox (loci of recombination) site called the LoxP sequence are derived from bacteriophage P1. The Cre protein specifically recognizes two LoxP sites within DNA and causes ...
The expression of Pdx1 in some lineage-labeled cells at E13.5 may be explained by the relatively stable nature of the Pdx1 protein translated prior to gene recombination. Figure 2 Impaired exocrine development in Elastase-Cre-mediated Pdx1-depleted mice. (A–P) Macroscopic and histological views...
Another approach to induce genome editing avoiding hormonal effects of tamoxifen that seems to be preferable in this application is the doxycycline activation system. Altogether, our present data cautions against interpreting lack of phenotype in Cre/Lox models as lack of effect of gene deletion, ...