这些小鼠可能对研究神经元分化有重要作用。 图11. 荧光检测PvalbCre+/-; Rosa26tdTomato+/-小鼠大脑皮层中间神经元tdTomato的表达。 图12. 荧光检测PvalbCre+/-; Rosa26tdTomato+/-小鼠海马中间神经元tdTomato的表达。结果显示:双阳性小鼠海马中间神经元有tdTomato的表达,表达类型符合预期。 如您有相关需求,欢迎...
NmuCre/+; Rosa26tdTomato/+双转基因鼠,可在小鼠大脑SNR(substantia nigra pars reticulate)区中检测 到tdTomato的表达. 图 3 NmuCre/+; Rosa26tdTomato/+小鼠各组织中tdTomato的表达情况.TdTomato在心,大肠,小肠,胰腺, 卵巢,子宫,胸腺,胃的部分细胞中表达,在棕色脂肪,肾,肺,肝,肌肉,唾液腺,皮肤,脾的...
4.安全位点敲入Cre小鼠模型:目前最新的Cre小鼠模型构建策略。为了避免基因随机插入可能导致诸多不利因素影响(比如表达位置影响作用及插入突变等),通过将Cre定点敲入到所谓的“安全”位点,比如Rosa26、Hprt和Hipp 11等位点。其中以Rosa26位点最为常用。 Rosa26位点特点,其本身含有基因转录的广泛启动子,形成mRNA,却无翻译...
In this study, we generated a tTA-dependent photoactivatable Cre-loxP recombinase knock-in mouse model (TRE-PA-Cre mice) using a CRISPR/Cas9 system. These mice were crossed with ROSA26-tdTomato mice (Cre reporter mouse) to visualize DNA recombination as marked by tdTomato expression. We ...
tdTomato was assessed on TCRβ+ CD4+ (red) and TCRβ+ CD8+ (green) T cells. (D) Lymphocytes were isolated from LN and spleen of ThPOKCreERT2.hCD2 Rosa26LSL.tdTomato mice that have received 10 consecutive intraperitoneal injections of tamoxifen (1 mg in 5% EtOH/sunflower oil) or ...
At 48 h post-transfection, a high level of tdTomato expres- sion was detected in all six cell lines, a pattern that was highly similar to the expression of the ubiquitous CMV promoter-driven tdTomato transgene (Figure 1C). Taken together, these data strongly suggest that the pRosa26 ...
tdTomato GFP YFP CFP RFP Confetti H2B-mCherry ROSA26是人和鼠里面的一个位置,可以理解为一个不表达蛋白的gene。 ROSA26 is a locus used for constitutive, ubiquitous gene expression in mice. It was first isolated in 1991 in a gene-trap mutagenesis screen of embryonic stem cells (ESCs). Over 13...
5). Also, we found that the mice injected with PA-Cre 3.0 showed higher recombination efficiency and tdTomato reporter expressions than the original PA-Cre. These results reveal that PA-Cre 3.0 is applicable for blue-light-dependent Cre-loxP recombination in the single loxP pair target in ...
CBLflox/flox [30], CBL-Bflox/flox, CD4-Cre [Tg(Cd4-cre)1Cwi/BfluJ] (The Jackson Laboratory), and Cre mT/mGFP reporter [Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J] (The Jackson Laboratory) strains were maintained on a C57/Bl6 background under specific pathogen-free conditions (Supp...
CRE-LOXP基因敲除系统(JACKSONLAB)