2.11. In vitro pull-down assay The expression of Myc-BAP1 was conducted referring to the previous protocol30. Briefly, the full length of BAP1 was cloned into pcDNA3.1 and transfected into HEK293F. Myc-BAP1 was purified with anti-Myc magnetic beads from cell lysates. Biotin-labeled PT33 ...
microRNAs (miR) are 20-to-24 nucleotides small noncoding RNAs, generated by Dicer processed ~ 70-base single-stranded RNA precursor [2,3,4], exert epigenetic regulation at transcription level by recognizing homologous mRNA sequence, which have been a research hotspot since first discovery in...
both in vitro and in tumor models. Osteopontin (OPN), a mediator associated with tumor progression in different tumor types, was among the top upregulated genes in both cells and tumors and was the most overexpressed gene coding for a secreted protein. We further show that Slug is a direct...