2.11. In vitro pull-down assay The expression of Myc-BAP1 was conducted referring to the previous protocol30. Briefly, the full length of BAP1 was cloned into pcDNA3.1 and transfected into HEK293F. Myc-BAP1 was purified with anti-Myc magnetic beads from cell lysates. Biotin-labeled PT33 ...
CRC development may take decades to transform into the final form of cancer. Many factors could modify the pace of this process, including diet, alcohol, and tobacco consumption, as well as contaminants present in water and food [5, 6]. Recently, the gut microbiota (GM) has also been ...
both in vitro and in tumor models. Osteopontin (OPN), a mediator associated with tumor progression in different tumor types, was among the top upregulated genes in both cells and tumors and was the most overexpressed gene coding for a secreted protein. We further show that Slug is a direct...