The following day, the medium was replaced with Stage 1 medium, changed daily for 3 days. For the final 5 days, used Stage 2 medium and refreshed daily. The detail components of mediums are as follows: Stage 1 medium: iCD1 medium77 containing Vitamin C (50 μg/ml, Sigma), bFGF (...
Mineral Mix (AIN-93G-MX, 94046) 48 g/kg, Calcium Phosphate, dibasic 3.4 g/kg, Vitamin Mix (AIN-93-VX, 94047) 21.0 g/kg, Choline Bitartrate 3.0 g/kg]) was fed to WT male mice and IDH2 KO male mice (8 weeks old) for 12 weeks (Doo Yeol Biotech, Seoul, South Kor...