The success of treating acute promyelocytic leukemia (APL) with all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) has revolutionized cancer therapy by inducing the differentiation of immature cancer cells. Differentiation therapy has greatly benefited patients withPML-RARAfusion, resulting in an...
Viruses and minimal vectors were produced by transfection of 293T cells using Polyethylenimine (PEI) (Sigma, Inc) as previously described [8]. Western blot analysis We used rabbit anti-TNPO3 antibody (ab71388, abcam), rabbit anti-CPSF6 antibody (Novus biological), human anti-p24 (NIBSC) and...
Rabbit Anti-HA Tag RB0411 Polyclonal Antibody abcepta Cat# AP1012a;RRID: AB_352510 Mouse monoclonal anti-Myc Sigma Cat# M4439;RRID: AB_439694 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma Cat# F1804;RRID: AB_262044 K48-linkage Specific Polyubiquitin (D9D5) Rabbit mAb Cell...
1:5000). Goat anti-mouse or anti-rabbit IgG secondary antibodies were coupled to Horseradish Peroxidase (HRP, Cwbiotech China), antibody complexes were detected using Pierce ECL Plus Western blotting substrate (Thermo Scientific). Chemiluminescence was detected using the ChemiDoc Imaging...