CR alters target profiles by increasing chromatin occupancy As RARγ regulates gene expression by direct DNA binding, we sought to address how CR fusions affect gene expression, which may disturb myeloid development and contribute to pre-leukemia transformation. We performed cleavage under targets and ...
Expression of the three CPSF6-358 fusion proteins was assessed by western blot with anti-HA antibody (Figure 4A). The HA epitope was also used to assess subcellular localization of the proteins by indirect immunofluorescence; CPSF6-358 was distributed throughout the cell, CPSF6-358-NLS was ...
Recombinant Anti-SYVN1/HRD1 antibody abcam Cat# ab170901;RRID: AB_2833021 Rabbit polyclonal anti-HRD1 Novus Cat# NB100-2526;RRID: AB_608700 Ubiquitin (P37) Antibody Cell Signaling Technology Cat# 58395S; RRID: AB_3075532 Ubiquitin Antibody Santa Cruz Biotechnology Cat# sc-271289;RRID: AB_106...
1:5000). Goat anti-mouse or anti-rabbit IgG secondary antibodies were coupled to Horseradish Peroxidase (HRP, Cwbiotech China), antibody complexes were detected using Pierce ECL Plus Western blotting substrate (Thermo Scientific). Chemiluminescence was detected using the ChemiDoc Imaging...