We used rabbit anti-TNPO3 antibody (ab71388, abcam), rabbit anti-CPSF6 antibody (Novus biological), human anti-p24 (NIBSC) and mouse anti-actin antibody (Sigma). The secondary antibodies were HRP-linked donkey anti-human IgG (Jackson), HRP-linked donkey anti-rabbit IgG and HRP-linked she...
1:5000). Goat anti-mouse or anti-rabbit IgG secondary antibodies were coupled to Horseradish Peroxidase (HRP, Cwbiotech China), antibody complexes were detected using Pierce ECL Plus Western blotting substrate (Thermo Scientific). Chemiluminescence was detected using the ChemiDoc Imaging...
Recombinant Anti-SYVN1/HRD1 antibody abcam Cat# ab170901;RRID: AB_2833021 Rabbit polyclonal anti-HRD1 Novus Cat# NB100-2526;RRID: AB_608700 Ubiquitin (P37) Antibody Cell Signaling Technology Cat# 58395S; RRID: AB_3075532 Ubiquitin Antibody Santa Cruz Biotechnology Cat# sc-271289;RRID: AB_106...
The success of treating acute promyelocytic leukemia (APL) with all-trans retinoic acid (ATRA) and arsenic trioxide (ATO) has revolutionized cancer therapy by inducing the differentiation of immature cancer cells. Differentiation therapy has greatly benefited patients withPML-RARAfusion, resulting in an...