Hartmann G & Krieg AM., 2000. Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J Immunol. 164(2):944-53.2- Krieg AM. et al., 1995. CpG motifs in bacterial DNA trigger direct B-cell activation. Nature. 374(6522):546-9.3- Hartmann G. et al., ...
目的:研究合CpG序列的寡核苷酸(unmethylated CpG motif containing oligodeoxynucleotide,CpG ODN)对人肺腺上皮细胞β防御素-2(human β-defensin 2,hBD-2)表达的影响及其作用途径。方法:培养人肺腺上皮细胞A549.用不同浓度的CpG ODN进行干预。用RT-PCR法和Western Blot法检测刺激后A549细胞hBD-2 mRNA和蛋白表达...
[0042] 本申请涉及的词语缩写: [0043] CpG-ODN,unmethylated CpG motif-containing oligodeoxynucleotide(含非甲 基化CpG motif基序的寡脱氧核苷酸); [0044] CDN,cyclic di-nucleotide(环二核苷酸); [0045] PLAN-CGC,phospholipid bilayer-coated aluminum nanoparticle co- modified with CpG-ODN and CDN(表面覆盖...
1 CpG ODN抗感染免疫作用机制 1.1 CpG ODN结构基础 CpG基序的核心是胞嘧啶核苷酸(dC)和鸟嘌呤核苷酸(dG),CpGDNA 是否具有免疫活性关键取决于dC的第5位碳原子是否被甲基化,如果胞嘧啶发生甲基化,则其活性丧失,CpG基序的免疫刺激活性不仅依赖于其核心CpG二核苷酸,而且这两个核苷酸的种类和序列也不能随意改变,破坏或...
CpG寡脱氧核苷酸、包含其的免疫组合物及制备组合物并通过其刺激免疫反应的方法.pdf,在这里公开一种CpG寡脱氧核苷酸(CpG‑ODN),其是作为开发用于在不同物种中使用作为佐剂的有效免疫刺激。TLR9为CpG‑ODN的细胞受体,且目前开发的CpG‑ODN对兔子的TLR9具有低活性。本
cp GDNA重组质粒作为疫苗佐剂的生物安全性评价景志忠,蒙学莲,房永祥,窦永喜,陈国华,才学鹏(中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046)摘要:CpGmotif是一些以非甲基化的胞嘧啶、鸟嘌呤二核苷酸(CpG)为模体的寡聚脱氧核苷酸(oligo—deoxynucleotides,ODN)的免疫刺激序列,是经研究证实的...
cpg-odn,unmethylatedcpgmotif-containingoligodeoxynucleotide(含非甲基化cpgmotif基序的寡脱氧核苷酸); cdn,cyclicdi-nucleotide(环二核苷酸); plan-cgc,phospholipidbilayer-coatedaluminumnanoparticleco-modifiedwithcpg-odnandcdn(表面覆盖磷脂双分子层的cpg-odn/cdn共修饰铝纳米粒); ...
CpG—ODNsasvaccineadjuvantsincludingtheresearchhistory,thecomplexityofimmunostimulatoryCpGO·DNs, andthepre—clinicalandclinicalstudiesofCpG—ODNs. [Keywords] CpGmotif;CpG—ODNs;vaccineadjuvant 1 CpG—ODNs免疫刺激活性的研究历程 示脊椎动物免疫系统可能存在一种通过识别 DNA 关于核酸具有免疫刺激活性 的研究可以...
ODN 2007 is provided lyophilized and is available in two quantities. tlrl-2007-1 (formerly tlrl-podnb-1): 1 mg (141.65 nmol) lyophilized ODN 2007 1.5 ml sterile endotoxin-free water tlrl-2007-5 (formerly tlrl-podnb-5): 5 mg (708.25 nmol) lyophilized ODN 2007 10 ml sterile endot...
“D”-type ODNs (also referred to as “A” type) have a phosphodiester core flanked by phosphorothioate terminal nucleotides. They carry a single CpG motif flanked by palindromic sequences and are characterized bypoly Gtails at the 3′ and 5′ ends that facilitate the formation of concatamer...