This work presents the validation process of a 2D-correlation spectroscopy (COSY) NMR quantification of 2-hydroxypropyl-尾-cyclodextrin in plasma. Specificity, linearity, precision (repeatability and intermediate precision), trueness, limits of quantification (LOQs), and accuracy were used as validation ...
HHCOSY(Homonuclear Correlation Spectroscopy)是一种基于核磁共振(NMR)的二维谱技术,主要用于解析有机化合物分子内氢核(¹H)之间的耦合关系,从而揭示分子内部结构信息。其核心功能是通过检测相邻或近邻氢原子的耦合信号,帮助确定氢原子的连接顺序和空间排布,为复杂有机分子(如天然产物、药物分子等)...
Morris, Decoupling two- dimensional NMR spectroscopy in both dimensions: pure shift NOESY and COSY, Angew. Chem. Int. Ed. 51 (2012) 6460.J.A. Aguilar, A.A. Colbourne, J. Cassani, M. Nilsson, and G.A. Morris, Decoupling Two- Dimensional NMR Spectroscopy in Both Dimensions: Pure Shift...
Two-DimensionalNMR Supervisor:KunliuReporter:YaoxueDate:16-07-2019 1 OUTLINE 核磁共振里的一些基本概念同核位移相关谱(CorrelatedSpectroscopy,COSY)异核位移相关谱(HMQC,HSQC)长程核位移相关谱(HMBC)NOESY和ROESY 2 二维核磁谱的分类：COSY(同核):1H-1H,19F-19F 通过化学键的原子核耦合(...
NOESY:(nuclear overhauser effect spectroscopy),空间相近的氢核的关系1H-1H NOESY谱图类似于COSY谱,若两核间有NOE相关,谱图中出现交叉峰。与COSY谱不同在于,NOESY揭示的是质子与质子间在空间的相互接近关系,而无法测量核间距的大小。可推测分子的立体结构。 HMQC:(C-H COSY),C和H直接相关谱1JCH,归属直接相连...
NOESY: (nuclear overhauser effect spectroscopy)实验是在一维NOE实验的基础上增加一个固定延迟时间和第三脉冲来检测NOE和化学交换信息。1H-1HNOESY谱图类似于COSY谱,若两核间有NOE相关,谱图中出现交叉峰。与COSY谱不同在于,NOESY揭示的是质子与质子间在空间的相互接近关系,而无法测量...
核磁共振NMR一维、二维谱 热度: 2D NMR Experiments gCOSY (gradient COrrelation SpectroscopY): a two‐dimensional NMR technique where the cross‐peaks appear between protons that have 3‐ bond proton‐proton scalar coupling, 3 J HH ROESY (Rotating‐frame Overhauser Effect SpectroscopY): a two‐ ...
常见二维核磁的功能 二维核磁原理 2DNMRisadomainofFTandpulsedspectroscopy +1D+1D=2D 90ºpulse2Ddetectsignalstwice(before/aftercoupling)Sameas1Dexperiment Transfersbetweencoupledspins 二维核磁原理 准备期(preparation)：准备期(preparation)：主要任务使核处于热平衡状态或者在一次采样之(preparation)后使自旋恢复到...
H-H相关谱图。建议在小木虫上下载宁永成编写的波谱分析书，上面有很详细的介绍
常见二维核磁的功能 二维核磁原理 2DNMRisadomainofFTandpulsedspectroscopy +1D+1D=2D 90ºpulse2Ddetectsignalstwice(before/aftercoupling)Sameas1Dexperiment Transfersbetweencoupledspins 二维核磁原理 准备期(preparation)：准备期(preparation)：主要任务使核处于热平衡状态或者在一次采样之(preparation)后使自旋恢复到...