providing low-cost genome-wide chromatin maps. By simplifying library preparation CUT&Tag requires less than a day at the bench, from live cells to sequencing-ready barcoded libraries. As a result of low background levels, barcoded and pooled CUT&Tag libraries can be sequenced for as little...
M. et al. Hi-C: a comprehensive technique to capture the conformation of genomes. Methods 58, 268–276 (2012). Article CAS PubMed Google Scholar Li, G. et al. ChIA-PET tool for comprehensive chromatin interaction analysis with paired-end tag sequencing. Genome Biol. 11, R22 (2010). ...
We show how price elasticity can be estimated from income elasticity of demand. As an example, we use this approach to predict the cost and the effectiveness of using subsidies to increase the proportion of households owning toilets in rural India. An analytical relation between income elasticity ...
Whole-genome sequencingMolecular profilingPersonalized medicineHeterogeneityCost-effectiveness analysis (CEA) is widely used as a tool for prioritising health interventions, particularly in low-income and middle-income settings. However, among the major limitations of CEA are the omission of health-care ...
Sequencing is the third possibility for retrieving biological data, which is useful in case you don't find the data online for your organism or a closely related one. By the way, sequencing the gene of interest is always good practice to make sure you are working with what you think you ...
Genetic characteristics of blaOXA-48- and blaOXA-181-containing Klebsiella pneumoniae (EFN299), Klebsiella quasipneumoniae (EFN262), and Enterobacter cloacae (EFN743) isolated from clinical samples in a Ghanaian hospital were investigated by whole-genome sequencing. Transfer of plasmids by conjugation ...
The reference standard for Nocardia identification is established through genetic sequencing. The significant time investment required by these methods makes them inaccessible to many laboratories. Easy to use and ubiquitous in clinical labs, MALDI-TOF mass spectrometry, however, encounters an impediment ...
“It improved how we work in labs, how we analyze data, and how we run software. We have genome assembly experiments that went from taking 40 hours to being finished in 4 hours and RNA sequencing datasets where the processing time was reduced from 24 to just a few hours.”...
Collecting a large number of biological samples from free-ranging animals remains the overarching limitation for mammalian conservation genetics and molecular ecology. While great advances have been made in recent years, the focus has been on how to do more with less: deeper sequencing and fewer sam...
(dashed) arrows denote (indirect) activation and red blunt-end arrows indicate inhibiting activity.bDose dependence of pheromone activation for the indicated genes encoding pathway components. Shown are normalized RNA levels as measured in two independent RNA sequencing experiments (symbols with and ...