Agrobacterium-mediated transformation (AMT) is an indispensable tool in both plant and fungal biotechnology for transgene insertion into target cells1,2,3. By replacing the native oncogenic genes used by the bacterial pathogenAgrobacterium tumefacienswith user-defined DNA sequences, the innate DNA transf...
The Surface hydrophobicity/hydrophilicity distribution was calculated using the Pymol script (https://pymolwiki.org/index.php/Color_h). The DALI comparison server (http://ekhidna2.biocenter.helsinki.fi/dali) was used to search for and align structural homologues from the PDB. For sequence ...
114 were successfully re-sequenced. Within these 114 plasmids, 112 contained mutations relative to wild-type pMGT1 at 59 unique genetic loci. Interestingly, 73% of all mutations were localized within the RepA protein coding sequence of the pSC101 origin...