注意事项 试剂盒中染料(Dye)溶解后易氧化变质,需要现配现用,不建议储存。 温馨提示:本产品仅作科研实验使用,不支持临床等研究
试剂盒中染料(Dye)溶解后易氧化变质,需要现配现用,不建议储存。 温馨提示:本产品仅作科研实验使用,不支持临床等研究
Home > Trace elements > Cu / Copper assayRev.150627 Easy to use. For cell lysates, serum, plasma and wide variety of biological samples. Pre-preatment free! Species independent! No toxic substance! Applicable to microplate reader! [ Product Description ] Inside human body, approximately 95% o...
WST-1 was added 2 h prior to absorbance measurement at 450 nm using a Tecan GENios Plus microplate reader. For determination of cell viability, cells were plated in 6-well plates either in complete medium or serum-starved medium, and treated with vehicle [deionized H2O (diH2O) or ...
After incubation for 15 min, adding stop solution terminated the reaction, and the absorbance was measured at 450 nm using an automated microplate reader. 2.5. Antioxidant enzyme activity Determination of copper/zinc superoxide dismutase (Cu/Zn-SOD) activity was determined by the modification of the...
A BioTek ELx800 (USA) microplate reader was used to evaluate cell viability at 490 nm. EdU assay The U251 and LN229 glioma cell lines were seeded in 6-well plates and cultured with siRARRES2 for 48 h. Then, an EdU assay kit (Beyotime, China) was used to measure the effect of ...
Then, 100 μL of R3 stimulator was added to the designated positive control wells (H2O2) and incubated for 20 more minutes at 37 °C in the dark. The DCF staining buffer was carefully removed, and 100 μL of the prepared test buffer was added. A microplate reader was used to quantify...
Mitochondrial respiration was evaluated by a seahorse XF analyzer (Agilent XF96, USA). Typically, on the day following HepG2 cell seeding into Seahorse XF96 microplate (1 × 104cells per well), cells were incubated with Cu-I@BSA-EBGA (10 µg/mL Cu) or PBS for 6 h and further...
The OD value at 450 nm was detected using a microplate reader (BioTek Instruments, MQX 200). GSH/GSSG measurement After myocardial tissues were weighed, the GSH/GSSG ratio was determined using the Glutathione Reductase/5,5’-Dithiobis-(2-nitrobenzoic acid) (DTNB) Cycling Assay Kit (Beyotime...
The light absorbance at 490 nm was measured using Infinite M200 microplate reader (Tecan, Switzerland). Real-time cell proliferation assay The experiments were performed using xCELLigence biosensor cell analysis system (ACEA Biosciences, USA). For real-time monitoring of cell proliferation 5 ×...