注意事项 试剂盒中染料(Dye)溶解后易氧化变质,需要现配现用,不建议储存。 温馨提示:本产品仅作科研实验使用,不支持临床等研究
注意事项 试剂盒中染料(Dye)溶解后易氧化变质,需要现配现用,不建议储存。 温馨提示:本产品仅作科研实验使用,不支持临床等研究
Home > Trace elements > Cu / Copper assayRev.150627 Easy to use. For cell lysates, serum, plasma and wide variety of biological samples. Pre-preatment free! Species independent! No toxic substance! Applicable to microplate reader! [ Product Description ] Inside human body, approximately 95% o...
The absorbances at 450 nm were determined at 48 h after the elesclomol + CuCl2 treatment via a microplate reader (BioTek Instruments, USA). DMEM containing 10% CCK-8 reagent was used as a blank. Each experiment was repeated at least three times. MitoTracker Red staining The cells ...
Fluorescence intensities (excitation 540 nm; emission 590 nm) were recorded using a Perkin Elmer Wallac 1420 Victor2 Microplate Reader. Values were derived from the standard curve of each experiment and compared to the data obtained by mass spectrometry-based metabolomics, to calculate total and...
The light absorbance at 490 nm was measured using Infinite M200 microplate reader (Tecan, Switzerland). Real-time cell proliferation assay The experiments were performed using xCELLigence biosensor cell analysis system (ACEA Biosciences, USA). For real-time monitoring of cell proliferation 5 ×...
5 × 103cells/well HUVECs and TECs were seeded and cultured in an incubator (5% CO2) at 37 °C for 72 h with different hydrogel. Cell Counting Kit-8 (CCK-8) assay was used to determine the cellular viability by measuring the absorbance at 450 nm through the microplate reader at ...
Mitochondrial respiration was evaluated by a seahorse XF analyzer (Agilent XF96, USA). Typically, on the day following HepG2 cell seeding into Seahorse XF96 microplate (1 × 104cells per well), cells were incubated with Cu-I@BSA-EBGA (10 µg/mL Cu) or PBS for 6 h and further...
Cell viability assay Control and CuSO4 treated cells were plated in a 96-well microplate and allowed to grow. At the end of copper treatment, the supernatant was aspirated and 100 μL medium mixed with MTS reagent (Promega, WI, USA) were added to each well, according to the manufacturer’...
The soluble nucleic acids were quantified in the supernatants using the Quant-It TM dsDNA High-Sensitivity kit (ThermoFisher, Scientific) following the manufacturer's instructions. Fluorescence was measured at ex485/em520 nm using a microplate reader (FLUOstar Optima®, BMG Labtech). 2.8. LDH ...