The large superfamily of labdane-related diterpenoids is defined by the cyclization of linear geranylgeranyl pyrophosphate (GGPP), catalyzed by copalyl diphosphate synthases (CPSs) to form the basic decalin core, the copalyl diphosphates (CPPs). Three stereochemically distinct CPPs have been found in...
ent-Kaurene is synthesized by the two-stepcyclizationof geranylgeranyldiphosphate(GGDP) via the intermediate,copalyl diphosphate(CDP). Theenzymesthat catalyze these reactions are referred to as the A and B activities, respectively, ofent-kaurene synthase. However, a more logical nomenclature was propose...
1. Optimizing expression and purification of recombinant Salvia miltiorrhiza copalyl diphosphate synthase protein in E.coli and preparation of rabbit antiserum against SmCPS; 丹参柯巴基焦磷酸合酶基因的优化表达、纯化及抗体制备2) farnesyl phosphate synthase gene 法呢基焦磷酸合酶基因 例句>> ...
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SmCPSent catalyzes the cyclization of GGPP to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by SmKS. Then, SmKO catalyzes the three-step oxidation of ent-kaurene to ent-kaurenoic acid. Our results show that the fused enzyme SmKS-SmCPSent increases ent-kaurene ...
functional characterization of ent- copalyl diphosphate synthase, kaurene synthase and kaurene oxidase in the salvia miltiorrhiza gibberellin biosynthetic ... P Su,Y Tong,Q Cheng,... 被引量: 0发表: 2018年 GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their Regulation Highlights include the cloning of...
a cycloisomerization reactionwhich transforms the general diterpenoid precursor (E,E,E)-geranylgeranyl diphosphate (GGPP; 1; see Scheme 1) intoent-copalyl diphosphate (ent-CPP; 2). This reaction is cata-lyzed by ent-CPP synthases (CPSs), which are ubiquitouslyfound in all vascular plants, thus...
Prime editing (PE) is a versatile CRISPR-Cas based precise genome-editing platform widely used to introduce a range of possible base conversions in various organisms. However, no PE systems have been shown to induce heritable mutations in tobacco, nor in any other dicot. In this study, we ...
(GGPP) to the diterpene copalyl diphosphate, an example of terpene synthase-catalysed cyclization initiated by double-bond protonation, rather than by hydrolysis of the diphosphate ester. PP indicates a diphosphate moiety. 개시된 순환화가 diterpene copalyl 이인산염에 geranylgeranyl...
Summary Isoprenyl diphosphate synthases (IDSs) catalyze some of the most basic steps in terpene biosynthesis by producing the prenyl diphosphate precursors of each of the various terpenoid classes. Most plants investigated have distinct enzymes that produce the short-chain all- trans ( E ) prenyl ...