Characterisation of viruses comprises (1) converting viral RNA to cDNA; (2) amplifying the cDNA in presence of primers 1 and 2, from the region of sequence identity, using the polymerase chain reaction; (3) mapping the amplified DNA using various restriction enzymes, and (4) comparing the ...
extracting messenger RNA (mRNA) from cells and tissuesfirst strand synthesisviral reverse transcriptionsecond strand reactionsreverse transcriptase PCRheat‐stable DNA polymerases used in PCRFirst Strand SynthesisSecond Strand Reactionsdoi:10.1002/9781444313611.ch53Matthew R. Walker...
Angiotensin-converting enzyme (ACE) is an Zn(II)-containing dipeptidyl carboxypeptidase that converts angiotensin I to the potent vasoconstrictor, angiotensin II. Using oligonucleotide probes based on the amino acid sequence of mouse kidney ACE, cDNA encoding this protein has been isolated. One cDNA...
Roles of dicer-like and argonaute proteins in TAS-derived small interfering RNA-triggered DNA methylation Trans-acting small interfering RNAs (ta-siRNAs; TAS) emerge as a class of plant-specific small RNAs that are initiated from microRNA-mediated cleavage of T... L Wu,L Mao,Y Qi - 《Plan...
For example, the bacterial glycine riboswitch facilitates glycine breakdown by controlling the expression of three genes required for degradation in response to glycine binding3. Similar target-responsive RNA- and DNA-based molecular switches have potential utility in a variety of technological applications...
展开 关键词: Humans Brain Alzheimer Disease Case-Control Studies In Situ Nick-End Labeling Blotting, Western Caspase 1 DNA Damage RNA, Messenger Female DOI: 10.1097/00005072-199906000-00002 被引量: 144 年份: 1999 收藏 引用 批量引用 报错 分享 全部...
Human ICE LAP-6 polypeptides and DNA (RNA) encoding such ICE LAP-6 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such ICE LAP-6 for the treatment of a susceptibility to viral infection, tumorogenesis and to diseas...
Overlapping genomic clones containing the entire sequence of the human angiotensin I-converting enzyme (ACE) gene were isolated from a lamda phage human DNA library. This gene spans 21 kilobases (kb) and comprises 26 exons, ranging in size from 88 to 481 base pairs. Intron-exon boundaries were...
An important application of robotically spotted DNA microarrays is the monitoring of RNA expression levels1,2. A clear limitation of this technology is the relatively large amount of RNA that is required per hybridization as a result of low hybridization
HOW tRNA WORKS Each amino acid is encoded in mRNA by a specific sequence of 3 nucleotide bases called a codon tRNA reads the current codon and binds the appropriate amino acid to the growing protein Source: http://mandevillehigh.stpsb.org/2005site/teacher_sites/laura_decker/dna_and_rna_diag...