Characterisation of viruses comprises (1) converting viral RNA to cDNA; (2) amplifying the cDNA in presence of primers 1 and 2, from the region of sequence identity, using the polymerase chain reaction; (3) mapping the amplified DNA using various restriction enzymes, and (4) comparing the ...
For example, the bacterial glycine riboswitch facilitates glycine breakdown by controlling the expression of three genes required for degradation in response to glycine binding3. Similar target-responsive RNA- and DNA-based molecular switches have potential utility in a variety of technological applications...
An important application of robotically spotted DNA microarrays is the monitoring of RNA expression levels1,2. A clear limitation of this technology is the relatively large amount of RNA that is required per hybridization as a result of low hybridization
Recent advances in single-cell RNA sequencing have enabled investigators to obtain more comprehensive profiling in different cancer cells, and an increasing number of transcription factor candidates have been identified and characterized to improve the efficiency of cancer cell reprogramming [77,78,79]. ...
There are two ACE promoters, a somatic promoter localized 5′ to the first exon of the gene and a germinal intragenic promoter located 5′ to the specific testicular ACE messenger RNA (99,102,103). The two alternate promoters of ACE exhibit highly contrasting cell specificities as the som...
Expressed ECE was inhibited by phosphoramidon, but not by thiorphan. It also cleaved big ET-1 efficiently but not big ET-2 or big ET-3. Northern blot analysis revealed that ECE messenger RNA is widely expressed in many tissues. 展开 ...
known as fluorescence spectroscopy, measures the fluorescent light emitted by a sample. Another, known as Raman spectroscopy, is useful for detecting molecules, such asDNAandRNA, that do not fluoresce or emit light at very low intensities. Both types were used to develop this cellphone detector....
Transgenic expression of bacterial nitroreductase (NTR) enzymes sensitizes eukaryotic cells to prodrugs such as metronidazole (MTZ), enabling selective cell-ablation paradigms that have expanded studies of cell function and regeneration in vertebrates. H
Synechococcuscells cultivated in flasks shaken under 50 μmol photons/m2/s white fluorescent light at 30 °C. Cells in the log phase were harvested and resuspended to an OD730of 4. Actinomycin D (ActD, 5 μg/mL) was added to block de novo mRNA synthesis and total RNA was isolated...
This usually refers to genes, DNA, RNA, or protein, and is particularly useful in comparing genes and other sequences in proteins and other sequences within an organism or between organisms, looking at evolutionary relationships between organisms, and using the patterns that exist across DNA and ...