Both conventional polymerase chain reaction (cPCR) and quantitative PCR (qPCR) have been employed to detect predation in the field because of their sensitivity and reproducibility. However, to date, few studies have been used to comprehensively demonstrate which method is more sensitive and ...
Comparison of a quantitative real-time polymerase chain reaction (qPCR) with conventional PCR, bacterial culture and ELISA for detec- tion of Mycobacterium avium subsp. paratuberculosis infection in sheep showing pa- thology of Johne's disease. Springerplus 2, 45-53....
A quantitative real-time PCR (qPCR) assay employing IS900 gene specific primers of Mycobacterium avium subsp. parartuberculosis (MAP) was compared with conventional PCR, bacterial culture and enzyme-linked immunosorbent assay in 38 sheep showing granulomatous enteritis and lymphadenitis with and without...
Conventional qPCR using PANDAA primers lacking the 3′ ADR was compared to PANDAA for the four most common K103 probe-binding sites (001 to 004).Template 001 (blue circles), which does not contain probe-binding site sequence variation, was included as a reference in each experiment against ...
Experimental determination of the adventitious presence of GM material in non-GM maize fields is usually carried out by applying validated event-specific GM quantification methods, mainly real-time PCR (qPCR)16 to grain samples taken at different locations within the field of interest17,18. Allnutt...
solanacearum DNA. Of the 47 potentially infected field samples collected, both IpxC LAMP and quantitative polymerase chain reaction (PCR) detected R. solanacearum DNA in 90% of the samples, followed by conventional PCR (86%) and ELISA (75%). This IpxC LAMP assay is a promising diagnostic ...
By conventional PCR, the HLB infectious bacterium has identified in 11 sweet oranges samples (31%) in which an amplicon of 1174 bp was generated. While qPCR subsequently reported to quantify the titer of Ca. L.asiaticus in host plant. In the present study qPCR re...
IL-22BP gene expression was analyzed by quantitative reverse transcriptase–PCR (RT-qPCR; n ¼ 3). (b) Lipopolysaccharide (LPS) or LPS þ IFNg (interferon-g) was added at day 6 on human MDDCs. IL-22BP gene expression was analyzed by RT-qPCR after 24 h (n ¼ 2). (c) Rat ...
For Q-PCR analyses, CT (threshold cycle) values, representing the target transcript abundance in samples, were calculated by the FLUIDIGM Real-time PCR analysis software using default settings. To compensate for technical variations between qPCR runs, the inter-array calibrators (civ.dil.1, civ.di...
Cossu A and Levin RE ( 2014) Rapid conventional PCR and real-time-qP- CR detection of low number of Salmonella enterica from ground beef without enrichment. Food Biotechnol 28: 96-105.Cossu A, Levin RE. Rapid conventional PCR and real-time-qPCR detection of low numbers of Salmonella ...