To assess intestinal integrity of rde-1 sel-11 double mutants, we tested intestinal barrier function in C. elegans39. Animals were fed a blue food dye, and leakage of the dye from the intestine into the body cavity (Smurf phenotype) was observed microscopically. While single mutants were ...
L. R-Ras interacts with filamin a to maintain endothelial barrier function. J. Cell Physiol. 226, 2287–2296 (2011). Article CAS Google Scholar Shang, X. et al. R-Ras and Rac GTPase cross-talk regulates hematopoietic progenitor cell migration, homing, and mobilization. J. Biol. Chem. ...
restore gut mucosal barrier function in female lpr mice. a Percentage of Lactobacillus strains in the order Lactobacillales (n = 4 per group). b Level of endotoxin in the blood of 6-week-old lpr mice (n = 6 mice per group; **P < 0.01). c Level of endotoxin in the ...
De-identified lung tissue samples collected at autopsy were formalin-fixed, paraffin-embedded, cut into 5-μm sections, and mounted onto slides. Sections were deparaffinized in an ethanol series, stained with hematoxylin and eosin (H&E), dehydrated and coverslipped. We evaluated ALI using a hi...
Cells were allowed to spread for 1 h at 37 °C on fibronectin‐coated slides (20 ng mL−1) or polylysine slides (VWR International, Fontenay sous‐Bois, France), fixed in 4% paraformaldehyde, washed and permeabilized for 5 min with 0.2% Triton X‐100, and then washed with phosphate‐...
nuclei and anti-GFAP antibody (Dako, Carpinteria, CA) was used to stain astrocytes. Tissue sections were incubated overnight in a humidified chamber at 4°C with primary antibodies diluted with 1% BSA in PBS with 0.05% Tween-20. The slides were then rinsed in PBS and incubated with the...
The BVES-KO mice (C57BL/6N-Bvestm1.1(KOMP)Vlcg/ MbpMmucd) with all coding exons deleted were obtained from Mutant Mouse Resource & Research Centers, UC Davis and maintained in our barrier facility. All mice were maintained under standard con- ditions of constant temperature (72 ± 4 °...
This showed us that preserving the membrane barrier function by the fT3SS pore was mostly dependent on the second and third positions (the residues most exposed in the channel) and that leakage was promoted by substitutions to polar (S, T, N, Q) and small (A, G) residues, and by pro...
Apparatus for controlling access to a parking space which may utilize either a vehicle body or a tire access blocking barrier. In one embodiment a barrier is pivotally mounted on a parking space floor
device by the control device is dependent to a value that is dependent on the control information, wherein the control device is designed to provide at least one network-callable function to the external communication device via the communication interface so that the OPC-UA standard is ...