All primary porcine fibroblasts were cultured in high-glucose DMEM (HyClone) supplemented with 15% fetal bovine serum (Gibco), 1× Non-Essential Amino Acids (Gibco), 1× GlutaMax (Gibco), 1× sodium pyruvate (Gibco), and 1× penicillin-streptomycin (Gibco) and maintained in a cell ...
E14Tg2a embryonic stem cells were maintained in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 15% fetal calf serum (FCS), 2 mM L-glutamine, 1 mM sodium pyruvate, 1% penicillin/streptomycin, 100 μM non-essential amino acids (all from Invitrogen), 100 μM β-merca...
Enteral nutrition and prevention of anastomotic leakage: A possible role for conditional essential amino acids? Results of a randomized clinical trialVan Barneveld, K W YBoelens, P GHeesakkers, F.F.B.MLuyer, M DBakker, J ARutten, H J T...
MEM Non-Essential Amino Acids Solution, 100X Gibco 11140050 Millex-HV Syringe Filter Unit, 0.45 µm, PVDF, 33 mm, gamma sterilized Millipore SLHV033RB Nalgene General Long-Term Storage Cryogenic Tubes Thermo Scientific 5000-1020 Nunc Cell Culture/Petri Dishes Thermo Scientific 171099 O...
Two constructs (N270/F271 and N273/R274) split BirA* in a way that separates the C-terminal domain that was previously described as essential for the activity of BirA15. Another construct segregates the N-terminal DNA-binding domain (Q65/L66), which was also reported to stimulate activity...
To our knowledge, the ponA-inducible expression system described here is the first system in which expression of all mature HCV proteins can be regulated in nontransformed human hepatocytes. Because expression of the HCV viral polyprotein is an essential early step in the HCV life cycle and ...
Caspase-8 is intimately involved in two essential death pathways—apoptosis and necroptosis—that are responsible for the fate of a cell. Stimulation of a death receptor (DR), such as Fas or tumor necrosis factor (TNF) receptor 1, by its ligand promotes recruitment of Fas-associated death doma...
The crucial role of mitochondrial protein import is illustrated by data from yeast [58] reporting that 5 genes are essential for mitochondrial viability, and the products of each of these genes are components of the mitochondrial protein import machinery. Included in this list are chaperonin 60 ...
The gRNA has been found to play essential roles in CRISPR systems, thus representing an attractive target to chemical manipulation17,27,28,29. In a previous study, Bhatia and co-workers have developed a robust light-activated CRISPR system30. However, to perform such regulation, additional protec...
The microbial cell wall is essential for maintenance of cell shape and resistance to external stressors1. The primary structural component of the cell wall is peptidoglycan, a glycopolymer with peptide crosslinks located outside of the cell membrane1. Peptidoglycan biosynthesis and structure are respons...